nfkb-p65

 

Enhancing the Analytical toolbox to analyze co-purifying Host Cell Proteins presence: N-(4)-(β-acetylglucosaminyl)-L-asparaginase case research

 

 

Ranges of host cell proteins (HCP) in purification intermediates and drug substances (DS) of monoclonal antibodies (mAb) should be rigorously monitored for the manufacturing of secure and efficacious biotherapeutics. In the course of the improvement of mAb1, an IgG1 product, sudden outcomes generated with HCP ELISA equipment triggered an investigation which led to the identification of a co-purifying HCP known as N-(4)-(β-acetylglucosaminyl)-L-asparaginase (AGA, EC3.5.1.26) by LC-MS/MS.

The danger evaluation carried out indicated a low immunogenicity danger for the co-purifying HCP and an ad-hoc stability research demonstrated no mAb glycan cleavage and thus no influence on product high quality.

 

 

DOCK2 {couples} with LEF-1 to control B cell metabolism and reminiscence

 

Dedicator of cytokinesis 2 (DOCK2) is important for the B cell differentiation, BCR signaling and humoral immune response.

 

 

Fractionation research carried out on sharpening steps revealed that AGA was co-eluted with the mAb. Very apparently, the native digestion protocol carried out to go deeper within the MS HCP profiling was discovered to be incompatible with correct AGA detection in final purification intermediate and DS, additional suggesting a hitchhiking conduct of AGA. In silico floor characterization of AGA additionally helps this speculation. Lastly, the mixed assist of HCP ELISA outcomes and mass spectrometry allowed course of optimization and removing of this co-purifying HCP. This text is protected by copyright. All rights reserved.
Key phrases: AGA N-(4)-(β-acetylglucosaminyl)-L-asparaginase; HCP ELISA assay; HCP identification and quantitation by LC-MS/MS; Hitchhiking conduct; Host Cell Proteins; Threat evaluation.

 

Nevertheless, the function of DOCK2 within the reminiscence response of B cell is unknown.

 

 

 

Through the use of two DOCK2 poor sufferers, we discovered that the reminiscence B cells have been decreased and the early activation of DOCK2 poor reminiscence B cells was abolished to the diploma of naïve B cells as a result of decreased expression of CD19 and CD21 mechanistically.

 

 

 

 

 

Apparently the expression of LEF-1, a adverse regulator of CD21, was elevated in DOCK2 poor B cells.

 

 

 

 

 

This was linked to the elevated expression of HIF-1α and cell metabolism, which in flip affected the ER construction.

 

nfkb-p65

nfkb-p65

 

 

Lastly, the discount of reminiscence B cells in DOCK2 sufferers was as a result of elevated apoptosis, which may be associated with the elevated metabolism.

 

 

Identification of Retinal Ganglion Cells from β-III Stained Fluorescent Microscopic Photos

 

 

Optic nerve crush in mouse mannequin is broadly used for investigating the course following retinal ganglion cell (RGCs) damage.
Handbook cell counting from β-III tubulin stained microscopic photographs has been routinely carried out to watch RGCs after an optic nerve crush damage, however is time-consuming and susceptible to observer variability.

Anti-Dog RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524329-5mg 5mg
EUR 620

Anti-Dog RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524329-5x5mg 5x5mg
EUR 2550

Anti-Cat RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524396-5mg 5mg
EUR 620

Anti-Cat RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524396-5x5mg 5x5mg
EUR 2550

Red Blood Cells, Chicken IgM Fraction

GWB-94168D 5 mg Ask for price

Anti-Swine RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524203-5mg 5mg
EUR 620

Anti-Swine RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524203-5x5mg 5x5mg
EUR 2550

Anti-Human RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524352-5mg 5mg
EUR 620

Anti-Human RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524352-5x5mg 5x5mg
EUR 2550

Anti-Mouse RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524365-5mg 5mg
EUR 620

Anti-Mouse RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524365-5x5mg 5x5mg
EUR 2550

Anti-Bovine RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524315-5mg 5mg
EUR 620

Anti-Bovine RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524315-5x5mg 5x5mg
EUR 2550

Anti-Turkey RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524333-5mg 5mg
EUR 620

Anti-Turkey RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524333-5x5mg 5x5mg
EUR 2550

Anti-Chicken RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524198-5mg 5mg
EUR 620

Anti-Chicken RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524198-5x5mg 5x5mg
EUR 2550

Anti-Hamster RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524271-5mg 5mg
EUR 620

Anti-Hamster RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524271-5x5mg 5x5mg
EUR 2550

Anti-Guinea Pig RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524301-5mg 5mg
EUR 620

Anti-Guinea Pig RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524301-5x5mg 5x5mg
EUR 2550

Anti-Horse RBC, IgM Fraction, (Polyclonal) (rabbit IgG)

MBS524261-5mg 5mg
EUR 620

Anti-Horse RBC, IgM Fraction, (Polyclonal) (rabbit IgG)

MBS524261-5x5mg 5x5mg
EUR 2550

IgG Fraction of anti-Mouse IgM [H&L] [Rabbit]

SG006 5.0 mg
EUR 125

IgG Fraction of anti-Mouse IgM [H&L] [Rabbit]

MBS417924-5mg 5mg
EUR 275

IgG Fraction of anti-Mouse IgM u (mu chain specific) [Goat]

MBS417923-5mg 5mg
EUR 275

Peroxidase Conjugated IgG fraction of anti-Mouse IgM [H&L] [Goat]

MBS417936-5mg 5mg
EUR 300

Fluorescein Conjugated IgG fraction of anti-Mouse IgM [H&L] [Rabbit]

SG013 5.0 mg
EUR 200

Fluorescein Conjugated IgG fraction of anti-Mouse IgM [H&L] [Rabbit]

MBS417931-5mg 5mg
EUR 300

MAP Fraction

MAPF-A 1 x 100 µg
EUR 318.24

MAP Fraction

MAPF-C 5 x 100 µg
EUR 1012.96

BSA Fraction V

30-AB75 1 kg Ask for price
Description: Standard Grade Bovine Serum Albumin, Cohn Fraction V, (99% pure)

Rat Ig fraction

31R-1021 10 mg
EUR 138
Description: Rat Ig fraction control or blocking reagent

Rat Ig fraction

31R-1045 10 mg
EUR 91
Description: Purified Rat Ig fraction for use as a control or blocking reagent

Rat Ig fraction

AS10-923 10 mg
EUR 139

Rat Ig Fraction

MBS687022-10mg 10mg
EUR 155

Rat Ig Fraction

MBS687022-5x10mg 5x10mg
EUR 545

Rat Ig fraction

MBS5308176-10mg 10mg
EUR 235

Rat Ig fraction

MBS5308176-5x10mg 5x10mg
EUR 905

BSA Fraction V

MBS537210-1kg 1kg
EUR 3590

BSA Fraction V

MBS537210-5x1kg 5x1kg
EUR 16065

Goat Ig fraction

31R-1008 10 mg
EUR 136.8
Description: Goat Ig fraction control or blocking reagent

Goat Ig fraction

31R-1032 10 mg
EUR 115
Description: Purified Goat Ig fraction for use as a control or blocking reagent

Goat Ig fraction

AS10-874 10 mg
EUR 113

Goat Ig Fraction

MBS687008-10mg 10mg
EUR 135

Goat Ig Fraction

MBS687008-5x10mg 5x10mg
EUR 455

EGF IgG fraction

MBS352089-01mL 0.1mL
EUR 725

EGF IgG fraction

MBS352089-5x01mL 5x0.1mL
EUR 3190

Goat Ig fraction

MBS5308117-10mg 10mg
EUR 225

Goat Ig fraction

MBS5308117-5x10mg 5x10mg
EUR 865

Horse Ig fraction

31R-1005 10 mg
EUR 136.8
Description: Horse Ig fraction control or blocking reagent

Sheep Ig fraction

31R-1023 10 mg
EUR 136.8
Description: Sheep Ig fraction control or blocking reagent

Horse Ig fraction

31R-1029 10 mg
EUR 92
Description: Purified Horse Ig fraction for use as a control or blocking reagent

Mouse Ig fraction

31R-1040 10 mg
EUR 128
Description: Purified Mouse Ig fraction for use as a control or blocking reagent

Sheep Ig fraction

31R-1047 10 mg
EUR 92
Description: Purified Sheep Ig fraction for use as a control or blocking reagent

Mouse Ig Fraction

GWB-7A4FD1 10 mg Ask for price

Horse Ig fraction

AS10-866 10 mg
EUR 113

Mouse Ig fraction

AS10-911 10 mg
EUR 139

Sheep Ig fraction

AS10-927 10 mg
EUR 139

Llama Ig Fraction

AS15-2842 10 mg
EUR 146

Llama Ig Fraction

MBS689513-10mg 10mg
EUR 130

Llama Ig Fraction

MBS689513-5x10mg 5x10mg
EUR 445

Horse Ig Fraction

MBS687004-10mg 10mg
EUR 135

Horse Ig Fraction

MBS687004-5x10mg 5x10mg
EUR 455

Mouse Ig Fraction

MBS687017-10mg 10mg
EUR 170

Mouse Ig Fraction

MBS687017-5x10mg 5x10mg
EUR 625

Sheep Ig Fraction

MBS687024-10mg 10mg
EUR 135

Sheep Ig Fraction

MBS687024-5x10mg 5x10mg
EUR 455

LHRH IgG fraction

MBS352152-01mL 0.1mL
EUR 680

LHRH IgG fraction

MBS352152-5x01mL 5x0.1mL
EUR 2985

Horse Ig fraction

MBS5308129-10mg 10mg
EUR 200

Horse Ig fraction

MBS5308129-5x10mg 5x10mg
EUR 750
This paper describes an automated approach for RGC identification. We developed and validated (i) a delicate cell candidate segmentation scheme and (ii) a classifier that eliminated false positives whereas retaining true positives.
Two main contributions have been made in cell candidate segmentation. First, a homomorphic filter was designed to regulate for the inhomogeneous illumination attributable to uneven penetration of β-III tubulin antibody.
Second, the optimum segmentation parameters for cell detection are extremely image-specific.
To handle this problem, we launched an offline-online parameter tuning strategy.
Offline tuning optimized mannequin parameters primarily based on coaching photographs and on-line tuning additional optimized the parameters on the testing stage without having entry to the bottom fact.
Within the cell identification stage, 31 geometric, statistical and textural options have been extracted from every segmented cell candidate, which was subsequently categorised as true or false positives by assist vector machine.
The homomorphic filter and the web parameter tuning strategy collectively elevated cell recall by 28%.
The complete pipeline attained a recall, precision and coefficient of dedication (r2) of 85.3%, 97.1% and 0.994.

CD48 Antibody (Pan Leukocyte Marker)

V9323-20UG 20ug
EUR 153.3
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V9323SAF-100UG 100ug
EUR 349.3
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V3016-100UG 100 ug
EUR 424.15
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V3016-20UG 20 ug
EUR 186.15
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V3016SAF-100UG 100 ug
EUR 424.15
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V3017-100UG 100 ug
EUR 424.15
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of the markers for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V3017-20UG 20 ug
EUR 186.15
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of the markers for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V3017SAF-100UG 100 ug
EUR 424.15
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of the markers for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V4698-100UG 100ug
EUR 363.3
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V4698-20UG 20ug
EUR 160.3
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V4698SAF-100UG 100ug
EUR 363.3
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V4699-100UG 100ug
EUR 363.3
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V4699-20UG 20ug
EUR 160.3
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V4699SAF-100UG 100ug
EUR 363.3
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V4742-100UG 100ug
EUR 363.3
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V4742-20UG 20ug
EUR 160.3
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V4742SAF-100UG 100ug
EUR 363.3
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V4743-100UG 100ug
EUR 363.3
Description: CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium.

CD48 Antibody (Pan Leukocyte Marker)

V4743-20UG 20ug
EUR 160.3
Description: CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium.

CD48 Antibody (Pan Leukocyte Marker)

V4743SAF-100UG 100ug
EUR 363.3
Description: CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium.

CD48 Antibody (Pan Leukocyte Marker)

V4744-100UG 100ug
EUR 363.3
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V4744-20UG 20ug
EUR 160.3
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V4744SAF-100UG 100ug
EUR 363.3
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody

MBS439057-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody

MBS439057-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody

MBS439057-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody

MBS439057-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody

MBS439057-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody

MBS439328-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody

MBS439328-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody

MBS439328-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody

MBS439328-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody

MBS439328-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4783]

MBS4382413-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4783]

MBS4382413-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4783]

MBS4382413-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4783]

MBS4382413-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4783]

MBS4382413-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4784]

MBS4382414-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4784]

MBS4382414-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4784]

MBS4382414-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4784]

MBS4382414-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4784]

MBS4382414-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4785]

MBS4382415-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4785]

MBS4382415-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4785]

MBS4382415-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4785]

MBS4382415-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4785]

MBS4382415-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4786]

MBS4382416-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4786]

MBS4382416-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4786]

MBS4382416-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4786]

MBS4382416-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4786]

MBS4382416-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4787]

MBS4382417-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4787]

MBS4382417-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4787]

MBS4382417-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4787]

MBS4382417-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD48 (Pan Leukocyte Marker) Mouse Monoclonal Antibody [Clone CD48/4787]

MBS4382417-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

Monoclonal CD48 (Pan Leukocyte Marker) Antibody - With BSA and Azide, Clone: 5-4.8

AMM01590G 0.05mg
EUR 475.2
Description: A Monoclonal antibody against Human CD48 (Pan Leukocyte Marker) - With BSA and Azide. The antibodies are raised in Mouse and are from clone 5-4.8. This antibody is applicable in IF, FC

Monoclonal CD48 (Pan Leukocyte Marker) Antibody - With BSA and Azide, Clone: 156-4H9

AMM01588G 0.05mg
EUR 475.2
Description: A Monoclonal antibody against Human CD48 (Pan Leukocyte Marker) - With BSA and Azide. The antibodies are raised in Mouse and are from clone 156-4H9. This antibody is applicable in IF, FC

Monoclonal CD48 (Pan Leukocyte Marker) Antibody - Without BSA and Azide, Clone: 5-4.8

AMM01591G 0.1mg
EUR 580.8
Description: A Monoclonal antibody against Human CD48 (Pan Leukocyte Marker) - Without BSA and Azide. The antibodies are raised in Mouse and are from clone 5-4.8. This antibody is applicable in IF, FC

Monoclonal CD48 (Pan Leukocyte Marker) Antibody - Without BSA and Azide, Clone: 156-4H9

AMM01589G 0.1mg
EUR 580.8
Description: A Monoclonal antibody against Human CD48 (Pan Leukocyte Marker) - Without BSA and Azide. The antibodies are raised in Mouse and are from clone 156-4H9. This antibody is applicable in IF, FC

Leukocyte Esterase, Human Leukocytes

MBS173116-1mL 1mL
EUR 245

Leukocyte Esterase, Human Leukocytes

MBS173116-25mL 25mL
EUR 1585

Leukocyte Esterase, Human Leukocytes

MBS173116-5mL 5mL
EUR 530

Leukocyte Esterase, Human Leukocytes

MBS173116-5x25mL 5x25mL
EUR 6920

Leukocyte protein

30-1168 5 ml
EUR 571
Description: Purified native Human Leukocyte protein

Leukocyte protein

MBS5303636-5mL 5mL
EUR 1015

Leukocyte protein

MBS5303636-5x5mL 5x5mL
EUR 4340

CD45RA (Leukocyte Marker)

MBS4381213-002mgWithBSAAzideat02mgmL 0.02mg(WithBSA&Azideat0.2mg/mL)
EUR 230

CD45RA (Leukocyte Marker)

MBS4381213-01mgWithBSAAzideat02mgmL 0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 405

CD45RA (Leukocyte Marker)

MBS4381213-01mgWithoutBSAAzideat1mgmL 0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 405

CD45RA (Leukocyte Marker)

MBS4381213-5x01mgWithBSAAzideat02mgmL 5x0.1mg(WithBSA&Azideat0.2mg/mL)
EUR 1725

CD45RA (Leukocyte Marker)

MBS4381213-5x01mgWithoutBSAAzideat1mgmL 5x0.1mg(WithoutBSA&Azideat1mg/mL)
EUR 1725

Leukocyte Esterase protein

30-1167 1 mL
EUR 263
Description: Purified native Human Leukocyte Esterase protein

Leukocyte Esterase protein

MBS5303642-1mL 1mL
EUR 440

Leukocyte Esterase protein

MBS5303642-5x1mL 5x1mL
EUR 1755

CD100 (Leukocyte Semaphorin)

MBS601115-01mg 0.1mg
EUR 625

CD100 (Leukocyte Semaphorin)

MBS601115-5x01mg 5x0.1mg
EUR 2665

LEUKOCYTE ELASTASE, Antibody

GWB-D078BD 0.1 mg Ask for price

Recombinant Pan troglodytes Leukocyte immunoglobulin-like receptor subfamily A member 6 (LILRA6), partial

MBS1379729-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1350

Recombinant Pan troglodytes Leukocyte immunoglobulin-like receptor subfamily A member 6 (LILRA6), partial

MBS1379729-005mgMammalianCell 0.05mg(Mammalian-Cell)
EUR 1605

Recombinant Pan troglodytes Leukocyte immunoglobulin-like receptor subfamily A member 6 (LILRA6), partial

MBS1379729-05mgEColi 0.5mg(E-Coli)
EUR 1350

Recombinant Pan troglodytes Leukocyte immunoglobulin-like receptor subfamily A member 6 (LILRA6), partial

MBS1379729-05mgYeast 0.5mg(Yeast)
EUR 1605

Recombinant Pan troglodytes Leukocyte immunoglobulin-like receptor subfamily A member 6 (LILRA6), partial

MBS1379729-1mgEColi 1mg(E-Coli)
EUR 2180

Recombinant Pan troglodytes Leukocyte immunoglobulin-like receptor subfamily B member 5 (LILRB5), partial

MBS1396753-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1365

Recombinant Pan troglodytes Leukocyte immunoglobulin-like receptor subfamily B member 5 (LILRB5), partial

MBS1396753-005mgMammalianCell 0.05mg(Mammalian-Cell)
EUR 1620
The availability of the proposed pipeline will enable environment friendly, correct and reproducible RGC quantification required for assessing the loss of life/survival of RGCs in illness fashions.
Key phrases: Cell segmentation; Fluorescent microscopy; Homomorphic filter; On-line parameter tuning; Retinal ganglion cell (RGC)

 

Liriopesides B induces apoptosis and cell cycle arrest in human non‑small cell lung most cancers cells

 

Dedicator of cytokinesis 2 (DOCK2) is important for the B cell differentiation, BCR signaling and humoral immune response.
Nevertheless, the function of DOCK2 within the reminiscence response of B cell is unknown.
Through the use of two DOCK2 poor sufferers, we discovered that the reminiscence B cells have been decreased and the early activation of DOCK2 poor reminiscence B cells was abolished to the diploma of naïve B cells as a result of decreased expression of CD19 and CD21 mechanistically.
Apparently the expression of LEF-1, a adverse regulator of CD21, was elevated in DOCK2 poor B cells.
This was linked to the elevated expression of HIF-1α and cell metabolism, which in flip affected the ER construction.
Lastly, the discount of reminiscence B cells in DOCK2 sufferers was as a result of elevated apoptosis, which may be associated with the elevated metabolism.
Key phrases: B cell; DOCK2; LEF-1
B cell zone reticular cell microenvironments form CXCL13 gradient formation
By way of the formation of focus gradients, morphogens drive graded responses to extracellular alerts, thereby fine-tuning cell behaviors in advanced tissues.
Right here we present that the chemokine CXCL13 varieties each soluble and immobilized gradients.
Particularly, CXCL13+follicular reticular cells kind a small-world community of steerage constructions, with pc simulations and optimization evaluation predicting that immobilized gradients created by this community promote B cell trafficking.
In step with this prediction, imaging evaluation present that CXCL13 binds to extracellular matrix parts in situ, constraining its diffusion. CXCL13 solubilization requires the protease cathepsin B that cleaves CXCL13 right into a secure product.
Mice missing cathepsin B show aberrant follicular structure, a phenotype related to efficient B cell homing to however not inside lymph nodes.
Our knowledge thus recommend that reticular cells of the B cell zone generate microenvironments that form each immobilized and soluble CXCL13 gradients

 

Anti-Dog RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524329-5mg 5mg
EUR 620

Anti-Dog RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524329-5x5mg 5x5mg
EUR 2550

Anti-Cat RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524396-5mg 5mg
EUR 620

Anti-Cat RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524396-5x5mg 5x5mg
EUR 2550

Red Blood Cells, Chicken IgM Fraction

GWB-94168D 5 mg Ask for price

Anti-Swine RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524203-5mg 5mg
EUR 620

Anti-Swine RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524203-5x5mg 5x5mg
EUR 2550

Anti-Human RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524352-5mg 5mg
EUR 620

Anti-Human RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524352-5x5mg 5x5mg
EUR 2550

Anti-Mouse RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524365-5mg 5mg
EUR 620

Anti-Mouse RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524365-5x5mg 5x5mg
EUR 2550

Anti-Bovine RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524315-5mg 5mg
EUR 620

Anti-Bovine RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524315-5x5mg 5x5mg
EUR 2550

Anti-Turkey RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524333-5mg 5mg
EUR 620

Anti-Turkey RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524333-5x5mg 5x5mg
EUR 2550

Anti-Chicken RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524198-5mg 5mg
EUR 620

Anti-Chicken RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524198-5x5mg 5x5mg
EUR 2550

Anti-Hamster RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524271-5mg 5mg
EUR 620

Anti-Hamster RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524271-5x5mg 5x5mg
EUR 2550

Anti-Guinea Pig RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524301-5mg 5mg
EUR 620

Anti-Guinea Pig RBC, IgM Fraction, (Polyclonal) (rabbit IgM)

MBS524301-5x5mg 5x5mg
EUR 2550

Anti-Horse RBC, IgM Fraction, (Polyclonal) (rabbit IgG)

MBS524261-5mg 5mg
EUR 620

Anti-Horse RBC, IgM Fraction, (Polyclonal) (rabbit IgG)

MBS524261-5x5mg 5x5mg
EUR 2550

IgG Fraction of anti-Mouse IgM [H&L] [Rabbit]

SG006 5.0 mg
EUR 125

IgG Fraction of anti-Mouse IgM [H&L] [Rabbit]

MBS417924-5mg 5mg
EUR 275

IgG Fraction of anti-Mouse IgM u (mu chain specific) [Goat]

MBS417923-5mg 5mg
EUR 275

Peroxidase Conjugated IgG fraction of anti-Mouse IgM [H&L] [Goat]

MBS417936-5mg 5mg
EUR 300

Fluorescein Conjugated IgG fraction of anti-Mouse IgM [H&L] [Rabbit]

SG013 5.0 mg
EUR 200

Fluorescein Conjugated IgG fraction of anti-Mouse IgM [H&L] [Rabbit]

MBS417931-5mg 5mg
EUR 300

MAP Fraction

MAPF-A 1 x 100 µg
EUR 318.24

MAP Fraction

MAPF-C 5 x 100 µg
EUR 1012.96

BSA Fraction V

30-AB75 1 kg Ask for price
Description: Standard Grade Bovine Serum Albumin, Cohn Fraction V, (99% pure)

Rat Ig fraction

31R-1021 10 mg
EUR 138
Description: Rat Ig fraction control or blocking reagent

Rat Ig fraction

31R-1045 10 mg
EUR 91
Description: Purified Rat Ig fraction for use as a control or blocking reagent

Rat Ig fraction

AS10-923 10 mg
EUR 139

Rat Ig Fraction

MBS687022-10mg 10mg
EUR 155

Rat Ig Fraction

MBS687022-5x10mg 5x10mg
EUR 545

Rat Ig fraction

MBS5308176-10mg 10mg
EUR 235

Rat Ig fraction

MBS5308176-5x10mg 5x10mg
EUR 905

BSA Fraction V

MBS537210-1kg 1kg
EUR 3590

BSA Fraction V

MBS537210-5x1kg 5x1kg
EUR 16065

Goat Ig fraction

31R-1008 10 mg
EUR 136.8
Description: Goat Ig fraction control or blocking reagent

Goat Ig fraction

31R-1032 10 mg
EUR 115
Description: Purified Goat Ig fraction for use as a control or blocking reagent

Goat Ig fraction

AS10-874 10 mg
EUR 113

Goat Ig Fraction

MBS687008-10mg 10mg
EUR 135

Goat Ig Fraction

MBS687008-5x10mg 5x10mg
EUR 455

EGF IgG fraction

MBS352089-01mL 0.1mL
EUR 725

EGF IgG fraction

MBS352089-5x01mL 5x0.1mL
EUR 3190

Goat Ig fraction

MBS5308117-10mg 10mg
EUR 225

Goat Ig fraction

MBS5308117-5x10mg 5x10mg
EUR 865

Horse Ig fraction

31R-1005 10 mg
EUR 136.8
Description: Horse Ig fraction control or blocking reagent

Sheep Ig fraction

31R-1023 10 mg
EUR 136.8
Description: Sheep Ig fraction control or blocking reagent

Horse Ig fraction

31R-1029 10 mg
EUR 92
Description: Purified Horse Ig fraction for use as a control or blocking reagent

Mouse Ig fraction

31R-1040 10 mg
EUR 128
Description: Purified Mouse Ig fraction for use as a control or blocking reagent

Sheep Ig fraction

31R-1047 10 mg
EUR 92
Description: Purified Sheep Ig fraction for use as a control or blocking reagent

Mouse Ig Fraction

GWB-7A4FD1 10 mg Ask for price

Horse Ig fraction

AS10-866 10 mg
EUR 113

Mouse Ig fraction

AS10-911 10 mg
EUR 139

Sheep Ig fraction

AS10-927 10 mg
EUR 139

Llama Ig Fraction

AS15-2842 10 mg
EUR 146

Llama Ig Fraction

MBS689513-10mg 10mg
EUR 130

Llama Ig Fraction

MBS689513-5x10mg 5x10mg
EUR 445

Horse Ig Fraction

MBS687004-10mg 10mg
EUR 135

Horse Ig Fraction

MBS687004-5x10mg 5x10mg
EUR 455

Mouse Ig Fraction

MBS687017-10mg 10mg
EUR 170

Mouse Ig Fraction

MBS687017-5x10mg 5x10mg
EUR 625

Sheep Ig Fraction

MBS687024-10mg 10mg
EUR 135

Sheep Ig Fraction

MBS687024-5x10mg 5x10mg
EUR 455

LHRH IgG fraction

MBS352152-01mL 0.1mL
EUR 680

LHRH IgG fraction

MBS352152-5x01mL 5x0.1mL
EUR 2985

Horse Ig fraction

MBS5308129-10mg 10mg
EUR 200

Horse Ig fraction

MBS5308129-5x10mg 5x10mg
EUR 750