nfkb-p65

 

Enhancing the Analytical toolbox to analyze co-purifying Host Cell Proteins presence: N-(4)-(β-acetylglucosaminyl)-L-asparaginase case research

 

 

Ranges of host cell proteins (HCP) in purification intermediates and drug substances (DS) of monoclonal antibodies (mAb) should be rigorously monitored for the manufacturing of secure and efficacious biotherapeutics. In the course of the improvement of mAb1, an IgG1 product, sudden outcomes generated with HCP ELISA equipment triggered an investigation which led to the identification of a co-purifying HCP known as N-(4)-(β-acetylglucosaminyl)-L-asparaginase (AGA, EC3.5.1.26) by LC-MS/MS.

The danger evaluation carried out indicated a low immunogenicity danger for the co-purifying HCP and an ad-hoc stability research demonstrated no mAb glycan cleavage and thus no influence on product high quality.

 

 

DOCK2 {couples} with LEF-1 to control B cell metabolism and reminiscence

 

Dedicator of cytokinesis 2 (DOCK2) is important for the B cell differentiation, BCR signaling and humoral immune response.

 

 

Fractionation research carried out on sharpening steps revealed that AGA was co-eluted with the mAb. Very apparently, the native digestion protocol carried out to go deeper within the MS HCP profiling was discovered to be incompatible with correct AGA detection in final purification intermediate and DS, additional suggesting a hitchhiking conduct of AGA. In silico floor characterization of AGA additionally helps this speculation. Lastly, the mixed assist of HCP ELISA outcomes and mass spectrometry allowed course of optimization and removing of this co-purifying HCP. This text is protected by copyright. All rights reserved.
Key phrases: AGA N-(4)-(β-acetylglucosaminyl)-L-asparaginase; HCP ELISA assay; HCP identification and quantitation by LC-MS/MS; Hitchhiking conduct; Host Cell Proteins; Threat evaluation.

 

Nevertheless, the function of DOCK2 within the reminiscence response of B cell is unknown.

 

 

 

Through the use of two DOCK2 poor sufferers, we discovered that the reminiscence B cells have been decreased and the early activation of DOCK2 poor reminiscence B cells was abolished to the diploma of naïve B cells as a result of decreased expression of CD19 and CD21 mechanistically.

 

 

 

 

 

Apparently the expression of LEF-1, a adverse regulator of CD21, was elevated in DOCK2 poor B cells.

 

 

 

 

 

This was linked to the elevated expression of HIF-1α and cell metabolism, which in flip affected the ER construction.

 

nfkb-p65

nfkb-p65

 

 

Lastly, the discount of reminiscence B cells in DOCK2 sufferers was as a result of elevated apoptosis, which may be associated with the elevated metabolism.

 

 

Identification of Retinal Ganglion Cells from β-III Stained Fluorescent Microscopic Photos

 

 

Optic nerve crush in mouse mannequin is broadly used for investigating the course following retinal ganglion cell (RGCs) damage.
Handbook cell counting from β-III tubulin stained microscopic photographs has been routinely carried out to watch RGCs after an optic nerve crush damage, however is time-consuming and susceptible to observer variability.

Rat Immunoglobulin M (IgM) ELISA Kit

DLR-IgM-Ra-48T 48T
EUR 511.2
Description: A sandwich quantitative ELISA assay kit for detection of Rat Immunoglobulin M (IgM) in samples from serum or plasma.

Rat Immunoglobulin M (IgM) ELISA Kit

DLR-IgM-Ra-96T 96T
EUR 658.8
Description: A sandwich quantitative ELISA assay kit for detection of Rat Immunoglobulin M (IgM) in samples from serum or plasma.

Rabbit Immunoglobulin M (IgM) ELISA Kit

DLR-IgM-Rb-48T 48T
EUR 609.6
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Immunoglobulin M (IgM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rabbit Immunoglobulin M (IgM) ELISA Kit

DLR-IgM-Rb-96T 96T
EUR 793.2
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Immunoglobulin M (IgM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-b-48Tests 48 Tests
EUR 567.6

Bovine Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-b-96Tests 96 Tests
EUR 784.8

Chicken Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Ch-48Tests 48 Tests
EUR 524.4

Chicken Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Ch-96Tests 96 Tests
EUR 723.6

Human Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Hu-48Tests 48 Tests
EUR 493.2

Human Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Hu-96Tests 96 Tests
EUR 678

Mouse Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Mu-48Tests 48 Tests
EUR 502.8

Mouse Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Mu-96Tests 96 Tests
EUR 693.6

Rat Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Ra-48Tests 48 Tests
EUR 524.4

Rat Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Ra-96Tests 96 Tests
EUR 723.6

Rabbit Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Rb-48Tests 48 Tests
EUR 640.8

Rabbit Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Rb-96Tests 96 Tests
EUR 890.4

Bovine Immunoglobulin M (IgM) ELISA Kit

RD-IgM-b-48Tests 48 Tests
EUR 543.6

Bovine Immunoglobulin M (IgM) ELISA Kit

RD-IgM-b-96Tests 96 Tests
EUR 750

Rat Immunoglobulin M (IgM) ELISA Kit

RD-IgM-Ra-48Tests 48 Tests
EUR 502.8

Rat Immunoglobulin M (IgM) ELISA Kit

RD-IgM-Ra-96Tests 96 Tests
EUR 692.4

Bovine Ig fraction

31R-1001 10 mg
EUR 136.8
Description: Bovine Ig fraction control or blocking reagent

Chicken Ig fraction

31R-1004 10 mg
EUR 248.4
Description: Chicken Ig fraction control or blocking reagent

Horse Ig fraction

31R-1005 10 mg
EUR 136.8
Description: Horse Ig fraction control or blocking reagent

Goat Ig fraction

31R-1008 10 mg
EUR 136.8
Description: Goat Ig fraction control or blocking reagent

Hamster Ig fraction

31R-1009 10 mg
EUR 165.6
Description: Hamster Ig fraction control or blocking reagent

Rabbit Ig fraction

31R-1018 10 mg
EUR 136.8
Description: Rabbit Ig fraction control or blocking reagent

Rat Ig fraction

31R-1021 10 mg
EUR 138
Description: Rat Ig fraction control or blocking reagent

Sheep Ig fraction

31R-1023 10 mg
EUR 136.8
Description: Sheep Ig fraction control or blocking reagent

Bovine Ig fraction

31R-1025 10 mg
EUR 136.8
Description: Purified Bovine Ig fraction for use as a control or blocking reagent

Chicken Ig fraction

31R-1028 10 mg
EUR 248.4
Description: Purified Chicken Ig fraction for use as a control or blocking reagent

Horse Ig fraction

31R-1029 10 mg
EUR 136.8
Description: Purified Horse Ig fraction for use as a control or blocking reagent

Goat Ig fraction

31R-1032 10 mg
EUR 163.2
Description: Purified Goat Ig fraction for use as a control or blocking reagent

Mouse Ig fraction

31R-1040 10 mg
EUR 180
Description: Purified Mouse Ig fraction for use as a control or blocking reagent

Rabbit Ig fraction

31R-1042 10 mg
EUR 136.8
Description: Purified Rabbit Ig fraction for use as a control or blocking reagent

Rat Ig fraction

31R-1045 10 mg
EUR 138
Description: Purified Rat Ig fraction for use as a control or blocking reagent

Sheep Ig fraction

31R-1047 10 mg
EUR 136.8
Description: Purified Sheep Ig fraction for use as a control or blocking reagent

BSA Fraction V

30-AB75 1 kg
EUR 1290
Description: Standard Grade Bovine Serum Albumin, Cohn Fraction V, (99% pure)

Mouse Anti-Meningococcal A/C/W/Y Oligosaccharides IgM (Combo) ELISA Kit, 96 tests, Quantitative

600-889-IGM 1 Kit
EUR 1074

Human Anti-Meningococcal Group A/C/W/Y Oligosaccharides IgM (Combo) ELISA Kit, 96 tests, Quantitative

600-884-IGM 1 Kit
EUR 1220.4

AFP antibody (Ig fraction)

70R-14345 1 ml
EUR 166.8
Description: Goat polyclonal AFP antibody (Ig fraction)

Guinea Pig Ig fraction

31R-1007 10 mg
EUR 138
Description: Guinea Pig Ig fraction control or blocking reagent

Guinea Pig Ig fraction

31R-1031 10 mg
EUR 165.6
Description: Purified Guinea Pig Ig fraction for use as a control or blocking reagent

FITC antibody (IgG fraction)

20C-CR1056SP 1 ml
EUR 289.2
Description: Rabbit polyclonal FITC antibody

WSE-7422 EzSubcell Fraction

2332338 1unit
EUR 477.6
Description: Fr act i onat i on of N ucl ei & M i t ochondr i a

Albumin, Cohn fraction pH5.2

AR2440 50g
EUR 180.06

Fraction Column Dufton 150mm

QFC112 EACH
EUR 121.09

Fraction Column Dufton 300mm

QFC122 EACH
EUR 135.27

Pyruvate Kinase antibody (IgG fraction)

20C-CR7017GP 1 mL
EUR 282
Description: Goat polyclonal Pyruvate Kinase antibody (IgG fraction)

Pyruvate Kinase antibody (IgG fraction)

20R-PG010 1 ml
EUR 282
Description: Goat polyclonal Pyruvate Kinase antibody (IgG fraction)

Fraction V BSA 10% solution

30R-AB022 2x10ml
EUR 232.8
Description: Bovine Serum Albumin, Crohn Fraction V 10% solution

BSA Fraction V (pH 5.2)

AK8245-0100 100g Ask for price

BSA Fraction V (pH 5.2)

AK8245-0500 500g Ask for price

BSA Fraction V (pH 5.2)

AK8245-1000 1kg Ask for price

BSA Fraction V (pH 7.0)

AK8905-0100 100g Ask for price

BSA Fraction V (pH 7.0)

AK8905-0500 500g Ask for price

BSA Fraction V (pH 7.0)

AK8905-1000 1kg Ask for price

Human Carcinogenicity, fraction soluble ST2

QY-E05507 96T
EUR 433.2

Sheep IgM(IgM)

QY-E140055 96T
EUR 495.6

BSA Cohn Fraction V (Microbiological Grade)

30-AB80 1 kg
EUR 1072.8
Description: Microbiological Grade Bovine Serum Albumin, Cohn Fraction V, (99% pure)

Fraction V Microbiological grade BSA powder

BAC61-0050 50gm
EUR 365.04

Fraction V Microbiological grade BSA powder

BAC61-0100 100gm
EUR 447.72

Fraction V Microbiological grade BSA powder

BAC61-0500 500gm
EUR 585

Fraction V Microbiological grade BSA powder

BAC61-1000 1KG Ask for price

Fraction V Microbiological grade BSA powder

BAC61-10000 10KG Ask for price

Fraction V BSA powder, pH 7

BAC62-0050 50gm
EUR 407.16

Fraction V BSA powder, pH 7

BAC62-0100 100gm
EUR 489.84

Fraction V BSA powder, pH 7

BAC62-0500 500gm
EUR 702

Fraction V BSA powder, pH 7

BAC62-1000 1KG
EUR 1014

Fraction V BSA powder, pH 7

BAC62-10000 10KG Ask for price

Fraction V BSA powder, pH 5.2

BAC63-0050 50gm
EUR 407.16

Fraction V BSA powder, pH 5.2

BAC63-0100 100gm
EUR 489.84

Fraction V BSA powder, pH 5.2

BAC63-0500 500gm
EUR 702
This paper describes an automated approach for RGC identification. We developed and validated (i) a delicate cell candidate segmentation scheme and (ii) a classifier that eliminated false positives whereas retaining true positives.
Two main contributions have been made in cell candidate segmentation. First, a homomorphic filter was designed to regulate for the inhomogeneous illumination attributable to uneven penetration of β-III tubulin antibody.
Second, the optimum segmentation parameters for cell detection are extremely image-specific.
To handle this problem, we launched an offline-online parameter tuning strategy.
Offline tuning optimized mannequin parameters primarily based on coaching photographs and on-line tuning additional optimized the parameters on the testing stage without having entry to the bottom fact.
Within the cell identification stage, 31 geometric, statistical and textural options have been extracted from every segmented cell candidate, which was subsequently categorised as true or false positives by assist vector machine.
The homomorphic filter and the web parameter tuning strategy collectively elevated cell recall by 28%.
The complete pipeline attained a recall, precision and coefficient of dedication (r2) of 85.3%, 97.1% and 0.994.

CD48 Antibody (Pan Leukocyte Marker)

V9316-100UG 100ug
EUR 499
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V9316-20UG 20ug
EUR 219
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V9316SAF-100UG 100ug
EUR 499
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V9323-100UG 100ug
EUR 499
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V9323-20UG 20ug
EUR 219
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

V9323SAF-100UG 100ug
EUR 499
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CD48 Antibody (Pan Leukocyte Marker)

RQ5270 100ul
EUR 419

CD48 Antibody (Pan Leukocyte Marker)

RQ5802 100 ug
EUR 419

Pan-PAX (Pan-PAX) Antibody

abx236136-100ug 100 ug
EUR 577.2

Monoclonal CD48 (Pan Leukocyte Marker) Antibody - With BSA and Azide, Clone: 156-4H9

AMM01588G 0.05mg
EUR 475.2
Description: A Monoclonal antibody against Human CD48 (Pan Leukocyte Marker) - With BSA and Azide. The antibodies are raised in Mouse and are from clone 156-4H9. This antibody is applicable in IF, FC

Monoclonal CD48 (Pan Leukocyte Marker) Antibody - Without BSA and Azide, Clone: 156-4H9

AMM01589G 0.1mg
EUR 580.8
Description: A Monoclonal antibody against Human CD48 (Pan Leukocyte Marker) - Without BSA and Azide. The antibodies are raised in Mouse and are from clone 156-4H9. This antibody is applicable in IF, FC

Monoclonal CD48 (Pan Leukocyte Marker) Antibody - With BSA and Azide, Clone: 5-4.8

AMM01590G 0.05mg
EUR 475.2
Description: A Monoclonal antibody against Human CD48 (Pan Leukocyte Marker) - With BSA and Azide. The antibodies are raised in Mouse and are from clone 5-4.8. This antibody is applicable in IF, FC

Monoclonal CD48 (Pan Leukocyte Marker) Antibody - Without BSA and Azide, Clone: 5-4.8

AMM01591G 0.1mg
EUR 580.8
Description: A Monoclonal antibody against Human CD48 (Pan Leukocyte Marker) - Without BSA and Azide. The antibodies are raised in Mouse and are from clone 5-4.8. This antibody is applicable in IF, FC

Leukocyte protein

30-1168 5 ml
EUR 601.2
Description: Purified native Human Leukocyte protein

Cytokeratin, pan (Epithelial Marker); Clone PAN-CK (Concentrate)

RA0430-C.5 0.5 ml
EUR 360

Leukocyte Esterase protein

30-1167 1 mL
EUR 315.6
Description: Purified native Human Leukocyte Esterase protein

H3 Pan Antibody

6806-50
EUR 379.2

LDH Pan antibody

10-1335 1 mg
EUR 310.8
Description: Mouse monoclonal LDH Pan antibody

LDH Pan antibody

10-1336 1 mg
EUR 310.8
Description: Mouse monoclonal LDH Pan antibody

SSX pan antibody

70R-50426 100 ul
EUR 292.8
Description: Purified Polyclonal SSX pan antibody

FOXO1 pan antibody

70R-37057 100 ug
EUR 392.4
Description: Rabbit Polyclonal FOXO1 pan antibody

PKC pan antibody

70R-33408 100 ug
EUR 392.4
Description: Rabbit polyclonal PKC pan antibody

NBPF pan antibody

70R-51031 100 ul
EUR 292.8
Description: Purified Polyclonal NBPF pan antibody

Synuclein pan antibody

70R-30766 100 ug
EUR 392.4
Description: Rabbit polyclonal Synuclein pan antibody

Actin pan antibody

70R-31095 100 ug
EUR 392.4
Description: Rabbit polyclonal Actin pan antibody

pan-AKT antibody

38602-100ul 100ul
EUR 302.4

Pan-Cadherin Antibody

48907-100ul 100ul
EUR 399.6

Pan-Cadherin Antibody

48907-50ul 50ul
EUR 286.8

pan Cadherin Antibody

45029-100ul 100ul
EUR 302.4

pan Cadherin Antibody

45029-50ul 50ul
EUR 224.4

pan Cadherin Antibody

45052-100ul 100ul
EUR 302.4

pan Cadherin Antibody

45052-50ul 50ul
EUR 224.4

pan-AKT Antibody

36716-100ul 100ul
EUR 302.4

pan CDH Antibody

37301-100ul 100ul
EUR 302.4

Keratin pan antibody

20R-2530 250 uL
EUR 612
Description: Rabbit polyclonal Keratin pan antibody

Synuclein-pan Antibody

33495-100ul 100ul
EUR 302.4

Synuclein-pan Antibody

33495-50ul 50ul
EUR 224.4

Actin-pan Antibody

33321-100ul 100ul
EUR 302.4

Actin-pan Antibody

33321-50ul 50ul
EUR 224.4

pan Endothelium antibody

10R-6386 100 ug
EUR 340.8
Description: Mouse monoclonal pan Endothelium antibody

pan Cadherin antibody

10R-7890 100 ug
EUR 386.4
Description: Mouse monoclonal pan Cadherin antibody

Actin-pan antibody

10R-A106a 100 ug
EUR 663.6
Description: Mouse monoclonal Actin-pan antibody

pan Cytokeratin antibody

10R-2060 100 ul
EUR 483.6
Description: Mouse monoclonal pan Cytokeratin antibody

Cytokeratin Pan antibody

10R-2096 100 ul
EUR 483.6
Description: Mouse monoclonal Cytokeratin Pan antibody

pan Keratin antibody

10R-2442 50 ug
EUR 486
Description: Mouse monoclonal pan Keratin antibody

pan-Cytokeratin antibody

23032-100ul 100ul
EUR 468

pan Cadherin Antibody

20-abx149140
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

pan-AKT Antibody

20-abx002260
  • EUR 493.20
  • EUR 710.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 50 ul

NBPF-pan Antibody

20-abx008107
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

SSX-pan Antibody

20-abx008681
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Pan-AKT Antibody

20-abx125375
  • EUR 710.40
  • EUR 1028.40
  • EUR 493.20
  • 100 ul
  • 200 ul
  • 50 ul

Actin-pan Antibody

20-abx013019
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Synuclein-pan Antibody

20-abx013202
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Pan pLDH Antibody

abx023860-1mg 1 mg
EUR 410.4

Pan pLDH Antibody

abx023861-1mg 1 mg
EUR 410.4

Pan SUMO Antibody

abx027085-400ul 400 ul
EUR 627.6

Pan SUMO Antibody

abx027085-80l 80 µl
EUR 343.2

Pan SUMO Antibody

abx027140-400ul 400 ul
EUR 627.6

Pan SUMO Antibody

abx027140-80l 80 µl
EUR 343.2

Actin- pan Antibody

ABF0115 100 ug
EUR 525.6

Actin- pan Antibody

ABF5288 100 ug
EUR 525.6

PKC- pan Antibody

ABF6197 100 ug
EUR 525.6

pan Cadherin Antibody

ABD2942 100 ug
EUR 525.6

pan- AKT Antibody

ABD7208 100 ug
EUR 525.6

pan Cadherin Antibody

ABD7514 100 ug
EUR 525.6

Pan myristoylation Antibody

20-abx322788
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Pan myristoylation Antibody

20-abx322789
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

pan Cadherin Antibody

abx217630-100ug 100 ug
EUR 526.8

Pan Ubiquitin Antibody

abx415347-50ug 50 ug
EUR 678

pan ADH Antibody

abx433090-200ul 200 ul
EUR 460.8

SHANK (pan) Antibody

abx440129-100ug 100 ug
EUR 693.6

SAPAP (pan) Antibody

abx440140-100ug 100 ug
EUR 693.6

SHANK (pan) Antibody

abx442377-100ug 100 ug
EUR 693.6

SAPAP (pan) Antibody

abx442388-100ug 100 ug
EUR 693.6

Ankyrin (pan) Antibody

abx442494-100ug 100 ug
EUR 693.6

SHANK (pan) Antibody

abx442658-100ug 100 ug
EUR 693.6

SAPAP (pan) Antibody

abx442669-100ug 100 ug
EUR 693.6

Ankyrin (pan) Antibody

abx442775-100ug 100 ug
EUR 693.6

SHANK (pan) Antibody

abx442938-100ug 100 ug
EUR 693.6

SAPAP (pan) Antibody

abx442949-100ug 100 ug
EUR 693.6

Ankyrin (pan) Antibody

abx443055-100ug 100 ug
EUR 693.6

SHANK (pan) Antibody

abx443218-100ug 100 ug
EUR 678

SAPAP (pan) Antibody

abx443229-100ug 100 ug
EUR 678

Ankyrin (pan) Antibody

abx443335-100ug 100 ug
EUR 678

SHANK (pan) Antibody

abx443499-100ug 100 ug
EUR 678

SAPAP (pan) Antibody

abx443510-100ug 100 ug
EUR 678

Ankyrin (pan) Antibody

abx443616-100ug 100 ug
EUR 678
The availability of the proposed pipeline will enable environment friendly, correct and reproducible RGC quantification required for assessing the loss of life/survival of RGCs in illness fashions.
Key phrases: Cell segmentation; Fluorescent microscopy; Homomorphic filter; On-line parameter tuning; Retinal ganglion cell (RGC)

 

Liriopesides B induces apoptosis and cell cycle arrest in human non‑small cell lung most cancers cells

 

Dedicator of cytokinesis 2 (DOCK2) is important for the B cell differentiation, BCR signaling and humoral immune response.
Nevertheless, the function of DOCK2 within the reminiscence response of B cell is unknown.
Through the use of two DOCK2 poor sufferers, we discovered that the reminiscence B cells have been decreased and the early activation of DOCK2 poor reminiscence B cells was abolished to the diploma of naïve B cells as a result of decreased expression of CD19 and CD21 mechanistically.
Apparently the expression of LEF-1, a adverse regulator of CD21, was elevated in DOCK2 poor B cells.
This was linked to the elevated expression of HIF-1α and cell metabolism, which in flip affected the ER construction.
Lastly, the discount of reminiscence B cells in DOCK2 sufferers was as a result of elevated apoptosis, which may be associated with the elevated metabolism.
Key phrases: B cell; DOCK2; LEF-1
B cell zone reticular cell microenvironments form CXCL13 gradient formation
By way of the formation of focus gradients, morphogens drive graded responses to extracellular alerts, thereby fine-tuning cell behaviors in advanced tissues.
Right here we present that the chemokine CXCL13 varieties each soluble and immobilized gradients.
Particularly, CXCL13+follicular reticular cells kind a small-world community of steerage constructions, with pc simulations and optimization evaluation predicting that immobilized gradients created by this community promote B cell trafficking.
In step with this prediction, imaging evaluation present that CXCL13 binds to extracellular matrix parts in situ, constraining its diffusion. CXCL13 solubilization requires the protease cathepsin B that cleaves CXCL13 right into a secure product.
Mice missing cathepsin B show aberrant follicular structure, a phenotype related to efficient B cell homing to however not inside lymph nodes.
Our knowledge thus recommend that reticular cells of the B cell zone generate microenvironments that form each immobilized and soluble CXCL13 gradients

 

Rat Immunoglobulin M (IgM) ELISA Kit

DLR-IgM-Ra-48T 48T
EUR 511.2
Description: A sandwich quantitative ELISA assay kit for detection of Rat Immunoglobulin M (IgM) in samples from serum or plasma.

Rat Immunoglobulin M (IgM) ELISA Kit

DLR-IgM-Ra-96T 96T
EUR 658.8
Description: A sandwich quantitative ELISA assay kit for detection of Rat Immunoglobulin M (IgM) in samples from serum or plasma.

Rabbit Immunoglobulin M (IgM) ELISA Kit

DLR-IgM-Rb-48T 48T
EUR 609.6
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Immunoglobulin M (IgM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rabbit Immunoglobulin M (IgM) ELISA Kit

DLR-IgM-Rb-96T 96T
EUR 793.2
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Immunoglobulin M (IgM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-b-48Tests 48 Tests
EUR 567.6

Bovine Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-b-96Tests 96 Tests
EUR 784.8

Chicken Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Ch-48Tests 48 Tests
EUR 524.4

Chicken Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Ch-96Tests 96 Tests
EUR 723.6

Human Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Hu-48Tests 48 Tests
EUR 493.2

Human Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Hu-96Tests 96 Tests
EUR 678

Mouse Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Mu-48Tests 48 Tests
EUR 502.8

Mouse Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Mu-96Tests 96 Tests
EUR 693.6

Rat Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Ra-48Tests 48 Tests
EUR 524.4

Rat Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Ra-96Tests 96 Tests
EUR 723.6

Rabbit Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Rb-48Tests 48 Tests
EUR 640.8

Rabbit Immunoglobulin M (IgM) ELISA Kit

RDR-IgM-Rb-96Tests 96 Tests
EUR 890.4

Bovine Immunoglobulin M (IgM) ELISA Kit

RD-IgM-b-48Tests 48 Tests
EUR 543.6

Bovine Immunoglobulin M (IgM) ELISA Kit

RD-IgM-b-96Tests 96 Tests
EUR 750

Rat Immunoglobulin M (IgM) ELISA Kit

RD-IgM-Ra-48Tests 48 Tests
EUR 502.8

Rat Immunoglobulin M (IgM) ELISA Kit

RD-IgM-Ra-96Tests 96 Tests
EUR 692.4

Bovine Ig fraction

31R-1001 10 mg
EUR 136.8
Description: Bovine Ig fraction control or blocking reagent

Chicken Ig fraction

31R-1004 10 mg
EUR 248.4
Description: Chicken Ig fraction control or blocking reagent

Horse Ig fraction

31R-1005 10 mg
EUR 136.8
Description: Horse Ig fraction control or blocking reagent

Goat Ig fraction

31R-1008 10 mg
EUR 136.8
Description: Goat Ig fraction control or blocking reagent

Hamster Ig fraction

31R-1009 10 mg
EUR 165.6
Description: Hamster Ig fraction control or blocking reagent

Rabbit Ig fraction

31R-1018 10 mg
EUR 136.8
Description: Rabbit Ig fraction control or blocking reagent

Rat Ig fraction

31R-1021 10 mg
EUR 138
Description: Rat Ig fraction control or blocking reagent

Sheep Ig fraction

31R-1023 10 mg
EUR 136.8
Description: Sheep Ig fraction control or blocking reagent

Bovine Ig fraction

31R-1025 10 mg
EUR 136.8
Description: Purified Bovine Ig fraction for use as a control or blocking reagent

Chicken Ig fraction

31R-1028 10 mg
EUR 248.4
Description: Purified Chicken Ig fraction for use as a control or blocking reagent

Horse Ig fraction

31R-1029 10 mg
EUR 136.8
Description: Purified Horse Ig fraction for use as a control or blocking reagent

Goat Ig fraction

31R-1032 10 mg
EUR 163.2
Description: Purified Goat Ig fraction for use as a control or blocking reagent

Mouse Ig fraction

31R-1040 10 mg
EUR 180
Description: Purified Mouse Ig fraction for use as a control or blocking reagent

Rabbit Ig fraction

31R-1042 10 mg
EUR 136.8
Description: Purified Rabbit Ig fraction for use as a control or blocking reagent

Rat Ig fraction

31R-1045 10 mg
EUR 138
Description: Purified Rat Ig fraction for use as a control or blocking reagent

Sheep Ig fraction

31R-1047 10 mg
EUR 136.8
Description: Purified Sheep Ig fraction for use as a control or blocking reagent

BSA Fraction V

30-AB75 1 kg
EUR 1290
Description: Standard Grade Bovine Serum Albumin, Cohn Fraction V, (99% pure)

Mouse Anti-Meningococcal A/C/W/Y Oligosaccharides IgM (Combo) ELISA Kit, 96 tests, Quantitative

600-889-IGM 1 Kit
EUR 1074

Human Anti-Meningococcal Group A/C/W/Y Oligosaccharides IgM (Combo) ELISA Kit, 96 tests, Quantitative

600-884-IGM 1 Kit
EUR 1220.4

AFP antibody (Ig fraction)

70R-14345 1 ml
EUR 166.8
Description: Goat polyclonal AFP antibody (Ig fraction)

Guinea Pig Ig fraction

31R-1007 10 mg
EUR 138
Description: Guinea Pig Ig fraction control or blocking reagent

Guinea Pig Ig fraction

31R-1031 10 mg
EUR 165.6
Description: Purified Guinea Pig Ig fraction for use as a control or blocking reagent

FITC antibody (IgG fraction)

20C-CR1056SP 1 ml
EUR 289.2
Description: Rabbit polyclonal FITC antibody

WSE-7422 EzSubcell Fraction

2332338 1unit
EUR 477.6
Description: Fr act i onat i on of N ucl ei & M i t ochondr i a

Albumin, Cohn fraction pH5.2

AR2440 50g
EUR 180.06

Fraction Column Dufton 150mm

QFC112 EACH
EUR 121.09

Fraction Column Dufton 300mm

QFC122 EACH
EUR 135.27

Pyruvate Kinase antibody (IgG fraction)

20C-CR7017GP 1 mL
EUR 282
Description: Goat polyclonal Pyruvate Kinase antibody (IgG fraction)

Pyruvate Kinase antibody (IgG fraction)

20R-PG010 1 ml
EUR 282
Description: Goat polyclonal Pyruvate Kinase antibody (IgG fraction)

Fraction V BSA 10% solution

30R-AB022 2x10ml
EUR 232.8
Description: Bovine Serum Albumin, Crohn Fraction V 10% solution

BSA Fraction V (pH 5.2)

AK8245-0100 100g Ask for price

BSA Fraction V (pH 5.2)

AK8245-0500 500g Ask for price

BSA Fraction V (pH 5.2)

AK8245-1000 1kg Ask for price

BSA Fraction V (pH 7.0)

AK8905-0100 100g Ask for price

BSA Fraction V (pH 7.0)

AK8905-0500 500g Ask for price

BSA Fraction V (pH 7.0)

AK8905-1000 1kg Ask for price

Human Carcinogenicity, fraction soluble ST2

QY-E05507 96T
EUR 433.2

Sheep IgM(IgM)

QY-E140055 96T
EUR 495.6

BSA Cohn Fraction V (Microbiological Grade)

30-AB80 1 kg
EUR 1072.8
Description: Microbiological Grade Bovine Serum Albumin, Cohn Fraction V, (99% pure)

Fraction V Microbiological grade BSA powder

BAC61-0050 50gm
EUR 365.04

Fraction V Microbiological grade BSA powder

BAC61-0100 100gm
EUR 447.72

Fraction V Microbiological grade BSA powder

BAC61-0500 500gm
EUR 585

Fraction V Microbiological grade BSA powder

BAC61-1000 1KG Ask for price

Fraction V Microbiological grade BSA powder

BAC61-10000 10KG Ask for price

Fraction V BSA powder, pH 7

BAC62-0050 50gm
EUR 407.16

Fraction V BSA powder, pH 7

BAC62-0100 100gm
EUR 489.84

Fraction V BSA powder, pH 7

BAC62-0500 500gm
EUR 702

Fraction V BSA powder, pH 7

BAC62-1000 1KG
EUR 1014

Fraction V BSA powder, pH 7

BAC62-10000 10KG Ask for price

Fraction V BSA powder, pH 5.2

BAC63-0050 50gm
EUR 407.16

Fraction V BSA powder, pH 5.2

BAC63-0100 100gm
EUR 489.84

Fraction V BSA powder, pH 5.2

BAC63-0500 500gm
EUR 702