Enhancing the Analytical toolbox to analyze co-purifying Host Cell Proteins presence: N-(4)-(β-acetylglucosaminyl)-L-asparaginase case research
Ranges of host cell proteins (HCP) in purification intermediates and drug substances (DS) of monoclonal antibodies (mAb) should be rigorously monitored for the manufacturing of secure and efficacious biotherapeutics. In the course of the improvement of mAb1, an IgG1 product, sudden outcomes generated with HCP ELISA equipment triggered an investigation which led to the identification of a co-purifying HCP known as N-(4)-(β-acetylglucosaminyl)-L-asparaginase (AGA, EC3.5.1.26) by LC-MS/MS.
The danger evaluation carried out indicated a low immunogenicity danger for the co-purifying HCP and an ad-hoc stability research demonstrated no mAb glycan cleavage and thus no influence on product high quality.
DOCK2 {couples} with LEF-1 to control B cell metabolism and reminiscence
Dedicator of cytokinesis 2 (DOCK2) is important for the B cell differentiation, BCR signaling and humoral immune response.
Fractionation research carried out on sharpening steps revealed that AGA was co-eluted with the mAb. Very apparently, the native digestion protocol carried out to go deeper within the MS HCP profiling was discovered to be incompatible with correct AGA detection in final purification intermediate and DS, additional suggesting a hitchhiking conduct of AGA. In silico floor characterization of AGA additionally helps this speculation. Lastly, the mixed assist of HCP ELISA outcomes and mass spectrometry allowed course of optimization and removing of this co-purifying HCP. This text is protected by copyright. All rights reserved.
Key phrases: AGA N-(4)-(β-acetylglucosaminyl)-L-asparaginase; HCP ELISA assay; HCP identification and quantitation by LC-MS/MS; Hitchhiking conduct; Host Cell Proteins; Threat evaluation.
Nevertheless, the function of DOCK2 within the reminiscence response of B cell is unknown.
Through the use of two DOCK2 poor sufferers, we discovered that the reminiscence B cells have been decreased and the early activation of DOCK2 poor reminiscence B cells was abolished to the diploma of naïve B cells as a result of decreased expression of CD19 and CD21 mechanistically.
Apparently the expression of LEF-1, a adverse regulator of CD21, was elevated in DOCK2 poor B cells.
This was linked to the elevated expression of HIF-1α and cell metabolism, which in flip affected the ER construction.

nfkb-p65
Lastly, the discount of reminiscence B cells in DOCK2 sufferers was as a result of elevated apoptosis, which may be associated with the elevated metabolism.
Identification of Retinal Ganglion Cells from β-III Stained Fluorescent Microscopic Photos
Optic nerve crush in mouse mannequin is broadly used for investigating the course following retinal ganglion cell (RGCs) damage.
Handbook cell counting from β-III tubulin stained microscopic photographs has been routinely carried out to watch RGCs after an optic nerve crush damage, however is time-consuming and susceptible to observer variability.
Rat Immunoglobulin M (IgM) ELISA Kit |
DLR-IgM-Ra-48T |
DL Develop |
48T |
EUR 511.2 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Immunoglobulin M (IgM) in samples from serum or plasma. |
Rat Immunoglobulin M (IgM) ELISA Kit |
DLR-IgM-Ra-96T |
DL Develop |
96T |
EUR 658.8 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Immunoglobulin M (IgM) in samples from serum or plasma. |
Rabbit Immunoglobulin M (IgM) ELISA Kit |
DLR-IgM-Rb-48T |
DL Develop |
48T |
EUR 609.6 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Immunoglobulin M (IgM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Rabbit Immunoglobulin M (IgM) ELISA Kit |
DLR-IgM-Rb-96T |
DL Develop |
96T |
EUR 793.2 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Immunoglobulin M (IgM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Bovine Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 567.6 |
Bovine Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 784.8 |
Chicken Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Ch-48Tests |
Reddot Biotech |
48 Tests |
EUR 524.4 |
Chicken Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Ch-96Tests |
Reddot Biotech |
96 Tests |
EUR 723.6 |
Human Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 493.2 |
Human Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 678 |
Mouse Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 502.8 |
Mouse Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 693.6 |
Rat Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 524.4 |
Rat Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 723.6 |
Rabbit Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Rb-48Tests |
Reddot Biotech |
48 Tests |
EUR 640.8 |
Rabbit Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Rb-96Tests |
Reddot Biotech |
96 Tests |
EUR 890.4 |
Bovine Immunoglobulin M (IgM) ELISA Kit |
RD-IgM-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 543.6 |
Bovine Immunoglobulin M (IgM) ELISA Kit |
RD-IgM-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 750 |
Rat Immunoglobulin M (IgM) ELISA Kit |
RD-IgM-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 502.8 |
Rat Immunoglobulin M (IgM) ELISA Kit |
RD-IgM-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 692.4 |
Bovine Ig fraction |
31R-1001 |
Fitzgerald |
10 mg |
EUR 136.8 |
Description: Bovine Ig fraction control or blocking reagent |
Chicken Ig fraction |
31R-1004 |
Fitzgerald |
10 mg |
EUR 248.4 |
Description: Chicken Ig fraction control or blocking reagent |
Horse Ig fraction |
31R-1005 |
Fitzgerald |
10 mg |
EUR 136.8 |
Description: Horse Ig fraction control or blocking reagent |
Goat Ig fraction |
31R-1008 |
Fitzgerald |
10 mg |
EUR 136.8 |
Description: Goat Ig fraction control or blocking reagent |
Hamster Ig fraction |
31R-1009 |
Fitzgerald |
10 mg |
EUR 165.6 |
Description: Hamster Ig fraction control or blocking reagent |
Rabbit Ig fraction |
31R-1018 |
Fitzgerald |
10 mg |
EUR 136.8 |
Description: Rabbit Ig fraction control or blocking reagent |
Rat Ig fraction |
31R-1021 |
Fitzgerald |
10 mg |
EUR 138 |
Description: Rat Ig fraction control or blocking reagent |
Sheep Ig fraction |
31R-1023 |
Fitzgerald |
10 mg |
EUR 136.8 |
Description: Sheep Ig fraction control or blocking reagent |
Bovine Ig fraction |
31R-1025 |
Fitzgerald |
10 mg |
EUR 136.8 |
Description: Purified Bovine Ig fraction for use as a control or blocking reagent |
Chicken Ig fraction |
31R-1028 |
Fitzgerald |
10 mg |
EUR 248.4 |
Description: Purified Chicken Ig fraction for use as a control or blocking reagent |
Horse Ig fraction |
31R-1029 |
Fitzgerald |
10 mg |
EUR 136.8 |
Description: Purified Horse Ig fraction for use as a control or blocking reagent |
Goat Ig fraction |
31R-1032 |
Fitzgerald |
10 mg |
EUR 163.2 |
Description: Purified Goat Ig fraction for use as a control or blocking reagent |
Mouse Ig fraction |
31R-1040 |
Fitzgerald |
10 mg |
EUR 180 |
Description: Purified Mouse Ig fraction for use as a control or blocking reagent |
Rabbit Ig fraction |
31R-1042 |
Fitzgerald |
10 mg |
EUR 136.8 |
Description: Purified Rabbit Ig fraction for use as a control or blocking reagent |
Rat Ig fraction |
31R-1045 |
Fitzgerald |
10 mg |
EUR 138 |
Description: Purified Rat Ig fraction for use as a control or blocking reagent |
Sheep Ig fraction |
31R-1047 |
Fitzgerald |
10 mg |
EUR 136.8 |
Description: Purified Sheep Ig fraction for use as a control or blocking reagent |
BSA Fraction V |
30-AB75 |
Fitzgerald |
1 kg |
EUR 1290 |
Description: Standard Grade Bovine Serum Albumin, Cohn Fraction V, (99% pure) |
Mouse Anti-Meningococcal A/C/W/Y Oligosaccharides IgM (Combo) ELISA Kit, 96 tests, Quantitative |
600-889-IGM |
Alpha Diagnostics |
1 Kit |
EUR 1074 |
Human Anti-Meningococcal Group A/C/W/Y Oligosaccharides IgM (Combo) ELISA Kit, 96 tests, Quantitative |
600-884-IGM |
Alpha Diagnostics |
1 Kit |
EUR 1220.4 |
AFP antibody (Ig fraction) |
70R-14345 |
Fitzgerald |
1 ml |
EUR 166.8 |
Description: Goat polyclonal AFP antibody (Ig fraction) |
Guinea Pig Ig fraction |
31R-1007 |
Fitzgerald |
10 mg |
EUR 138 |
Description: Guinea Pig Ig fraction control or blocking reagent |
Guinea Pig Ig fraction |
31R-1031 |
Fitzgerald |
10 mg |
EUR 165.6 |
Description: Purified Guinea Pig Ig fraction for use as a control or blocking reagent |
FITC antibody (IgG fraction) |
20C-CR1056SP |
Fitzgerald |
1 ml |
EUR 289.2 |
Description: Rabbit polyclonal FITC antibody |
WSE-7422 EzSubcell Fraction |
2332338 |
Atto |
1unit |
EUR 477.6 |
Description: Fr act i onat i on of N ucl ei & M i t ochondr i a |
Albumin, Cohn fraction pH5.2 |
AR2440 |
Bio Basic |
50g |
EUR 180.06 |
|
Pyruvate Kinase antibody (IgG fraction) |
20C-CR7017GP |
Fitzgerald |
1 mL |
EUR 282 |
Description: Goat polyclonal Pyruvate Kinase antibody (IgG fraction) |
Pyruvate Kinase antibody (IgG fraction) |
20R-PG010 |
Fitzgerald |
1 ml |
EUR 282 |
Description: Goat polyclonal Pyruvate Kinase antibody (IgG fraction) |
Fraction V BSA 10% solution |
30R-AB022 |
Fitzgerald |
2x10ml |
EUR 232.8 |
Description: Bovine Serum Albumin, Crohn Fraction V 10% solution |
BSA Fraction V (pH 5.2) |
AK8245-0100 |
Akron Biotech |
100g |
Ask for price |
BSA Fraction V (pH 5.2) |
AK8245-0500 |
Akron Biotech |
500g |
Ask for price |
BSA Fraction V (pH 5.2) |
AK8245-1000 |
Akron Biotech |
1kg |
Ask for price |
BSA Fraction V (pH 7.0) |
AK8905-0100 |
Akron Biotech |
100g |
Ask for price |
BSA Fraction V (pH 7.0) |
AK8905-0500 |
Akron Biotech |
500g |
Ask for price |
BSA Fraction V (pH 7.0) |
AK8905-1000 |
Akron Biotech |
1kg |
Ask for price |
BSA Cohn Fraction V (Microbiological Grade) |
30-AB80 |
Fitzgerald |
1 kg |
EUR 1072.8 |
Description: Microbiological Grade Bovine Serum Albumin, Cohn Fraction V, (99% pure) |
Fraction V Microbiological grade BSA powder |
BAC61-0050 |
Equitech |
50gm |
EUR 365.04 |
|
Fraction V Microbiological grade BSA powder |
BAC61-0100 |
Equitech |
100gm |
EUR 447.72 |
|
Fraction V Microbiological grade BSA powder |
BAC61-0500 |
Equitech |
500gm |
EUR 585 |
|
Fraction V Microbiological grade BSA powder |
BAC61-1000 |
Equitech |
1KG |
Ask for price |
|
Fraction V Microbiological grade BSA powder |
BAC61-10000 |
Equitech |
10KG |
Ask for price |
|
Fraction V BSA powder, pH 7 |
BAC62-0050 |
Equitech |
50gm |
EUR 407.16 |
|
Fraction V BSA powder, pH 7 |
BAC62-0100 |
Equitech |
100gm |
EUR 489.84 |
|
Fraction V BSA powder, pH 7 |
BAC62-0500 |
Equitech |
500gm |
EUR 702 |
|
Fraction V BSA powder, pH 7 |
BAC62-1000 |
Equitech |
1KG |
EUR 1014 |
|
Fraction V BSA powder, pH 7 |
BAC62-10000 |
Equitech |
10KG |
Ask for price |
|
Fraction V BSA powder, pH 5.2 |
BAC63-0050 |
Equitech |
50gm |
EUR 407.16 |
|
Fraction V BSA powder, pH 5.2 |
BAC63-0100 |
Equitech |
100gm |
EUR 489.84 |
|
Fraction V BSA powder, pH 5.2 |
BAC63-0500 |
Equitech |
500gm |
EUR 702 |
|
This paper describes an automated approach for RGC identification. We developed and validated (i) a delicate cell candidate segmentation scheme and (ii) a classifier that eliminated false positives whereas retaining true positives.
Two main contributions have been made in cell candidate segmentation. First, a homomorphic filter was designed to regulate for the inhomogeneous illumination attributable to uneven penetration of β-III tubulin antibody.
Second, the optimum segmentation parameters for cell detection are extremely image-specific.
To handle this problem, we launched an offline-online parameter tuning strategy.
Offline tuning optimized mannequin parameters primarily based on coaching photographs and on-line tuning additional optimized the parameters on the testing stage without having entry to the bottom fact.
Within the cell identification stage, 31 geometric, statistical and textural options have been extracted from every segmented cell candidate, which was subsequently categorised as true or false positives by assist vector machine.
The homomorphic filter and the web parameter tuning strategy collectively elevated cell recall by 28%.
The complete pipeline attained a recall, precision and coefficient of dedication (r2) of 85.3%, 97.1% and 0.994.
CD48 Antibody (Pan Leukocyte Marker) |
V9316-100UG |
NSJ Bioreagents |
100ug |
EUR 499 |
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2. |
CD48 Antibody (Pan Leukocyte Marker) |
V9316-20UG |
NSJ Bioreagents |
20ug |
EUR 219 |
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2. |
CD48 Antibody (Pan Leukocyte Marker) |
V9316SAF-100UG |
NSJ Bioreagents |
100ug |
EUR 499 |
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2. |
CD48 Antibody (Pan Leukocyte Marker) |
V9323-100UG |
NSJ Bioreagents |
100ug |
EUR 499 |
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2. |
CD48 Antibody (Pan Leukocyte Marker) |
V9323-20UG |
NSJ Bioreagents |
20ug |
EUR 219 |
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2. |
CD48 Antibody (Pan Leukocyte Marker) |
V9323SAF-100UG |
NSJ Bioreagents |
100ug |
EUR 499 |
Description: Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2. |
Pan-PAX (Pan-PAX) Antibody |
abx236136-100ug |
Abbexa |
100 ug |
EUR 577.2 |
|
Monoclonal CD48 (Pan Leukocyte Marker) Antibody - With BSA and Azide, Clone: 156-4H9 |
AMM01588G |
Leading Biology |
0.05mg |
EUR 475.2 |
Description: A Monoclonal antibody against Human CD48 (Pan Leukocyte Marker) - With BSA and Azide. The antibodies are raised in Mouse and are from clone 156-4H9. This antibody is applicable in IF, FC |
Monoclonal CD48 (Pan Leukocyte Marker) Antibody - Without BSA and Azide, Clone: 156-4H9 |
AMM01589G |
Leading Biology |
0.1mg |
EUR 580.8 |
Description: A Monoclonal antibody against Human CD48 (Pan Leukocyte Marker) - Without BSA and Azide. The antibodies are raised in Mouse and are from clone 156-4H9. This antibody is applicable in IF, FC |
Monoclonal CD48 (Pan Leukocyte Marker) Antibody - With BSA and Azide, Clone: 5-4.8 |
AMM01590G |
Leading Biology |
0.05mg |
EUR 475.2 |
Description: A Monoclonal antibody against Human CD48 (Pan Leukocyte Marker) - With BSA and Azide. The antibodies are raised in Mouse and are from clone 5-4.8. This antibody is applicable in IF, FC |
Monoclonal CD48 (Pan Leukocyte Marker) Antibody - Without BSA and Azide, Clone: 5-4.8 |
AMM01591G |
Leading Biology |
0.1mg |
EUR 580.8 |
Description: A Monoclonal antibody against Human CD48 (Pan Leukocyte Marker) - Without BSA and Azide. The antibodies are raised in Mouse and are from clone 5-4.8. This antibody is applicable in IF, FC |
Leukocyte protein |
30-1168 |
Fitzgerald |
5 ml |
EUR 601.2 |
Description: Purified native Human Leukocyte protein |
Cytokeratin, pan (Epithelial Marker); Clone PAN-CK (Concentrate) |
RA0430-C.5 |
ScyTek Laboratories |
0.5 ml |
EUR 360 |
Leukocyte Esterase protein |
30-1167 |
Fitzgerald |
1 mL |
EUR 315.6 |
Description: Purified native Human Leukocyte Esterase protein |
LDH Pan antibody |
10-1335 |
Fitzgerald |
1 mg |
EUR 310.8 |
Description: Mouse monoclonal LDH Pan antibody |
LDH Pan antibody |
10-1336 |
Fitzgerald |
1 mg |
EUR 310.8 |
Description: Mouse monoclonal LDH Pan antibody |
SSX pan antibody |
70R-50426 |
Fitzgerald |
100 ul |
EUR 292.8 |
Description: Purified Polyclonal SSX pan antibody |
FOXO1 pan antibody |
70R-37057 |
Fitzgerald |
100 ug |
EUR 392.4 |
Description: Rabbit Polyclonal FOXO1 pan antibody |
PKC pan antibody |
70R-33408 |
Fitzgerald |
100 ug |
EUR 392.4 |
Description: Rabbit polyclonal PKC pan antibody |
NBPF pan antibody |
70R-51031 |
Fitzgerald |
100 ul |
EUR 292.8 |
Description: Purified Polyclonal NBPF pan antibody |
Synuclein pan antibody |
70R-30766 |
Fitzgerald |
100 ug |
EUR 392.4 |
Description: Rabbit polyclonal Synuclein pan antibody |
Actin pan antibody |
70R-31095 |
Fitzgerald |
100 ug |
EUR 392.4 |
Description: Rabbit polyclonal Actin pan antibody |
pan-AKT antibody |
38602-100ul |
SAB |
100ul |
EUR 302.4 |
Pan-Cadherin Antibody |
48907-100ul |
SAB |
100ul |
EUR 399.6 |
Pan-Cadherin Antibody |
48907-50ul |
SAB |
50ul |
EUR 286.8 |
pan Cadherin Antibody |
45029-100ul |
SAB |
100ul |
EUR 302.4 |
pan Cadherin Antibody |
45029-50ul |
SAB |
50ul |
EUR 224.4 |
pan Cadherin Antibody |
45052-100ul |
SAB |
100ul |
EUR 302.4 |
pan Cadherin Antibody |
45052-50ul |
SAB |
50ul |
EUR 224.4 |
pan-AKT Antibody |
36716-100ul |
SAB |
100ul |
EUR 302.4 |
pan CDH Antibody |
37301-100ul |
SAB |
100ul |
EUR 302.4 |
Keratin pan antibody |
20R-2530 |
Fitzgerald |
250 uL |
EUR 612 |
Description: Rabbit polyclonal Keratin pan antibody |
Synuclein-pan Antibody |
33495-100ul |
SAB |
100ul |
EUR 302.4 |
Synuclein-pan Antibody |
33495-50ul |
SAB |
50ul |
EUR 224.4 |
Actin-pan Antibody |
33321-100ul |
SAB |
100ul |
EUR 302.4 |
Actin-pan Antibody |
33321-50ul |
SAB |
50ul |
EUR 224.4 |
pan Endothelium antibody |
10R-6386 |
Fitzgerald |
100 ug |
EUR 340.8 |
Description: Mouse monoclonal pan Endothelium antibody |
pan Cadherin antibody |
10R-7890 |
Fitzgerald |
100 ug |
EUR 386.4 |
Description: Mouse monoclonal pan Cadherin antibody |
Actin-pan antibody |
10R-A106a |
Fitzgerald |
100 ug |
EUR 663.6 |
Description: Mouse monoclonal Actin-pan antibody |
pan Cytokeratin antibody |
10R-2060 |
Fitzgerald |
100 ul |
EUR 483.6 |
Description: Mouse monoclonal pan Cytokeratin antibody |
Cytokeratin Pan antibody |
10R-2096 |
Fitzgerald |
100 ul |
EUR 483.6 |
Description: Mouse monoclonal Cytokeratin Pan antibody |
pan Keratin antibody |
10R-2442 |
Fitzgerald |
50 ug |
EUR 486 |
Description: Mouse monoclonal pan Keratin antibody |
pan-Cytokeratin antibody |
23032-100ul |
SAB |
100ul |
EUR 468 |
pan Cadherin Antibody |
20-abx149140 |
Abbexa |
|
|
|
pan-AKT Antibody |
20-abx002260 |
Abbexa |
-
EUR 493.20
-
EUR 710.40
-
EUR 376.80
|
|
|
NBPF-pan Antibody |
20-abx008107 |
Abbexa |
-
EUR 360.00
-
EUR 526.80
-
EUR 226.80
|
|
|
SSX-pan Antibody |
20-abx008681 |
Abbexa |
-
EUR 360.00
-
EUR 526.80
-
EUR 226.80
|
|
|
Pan-AKT Antibody |
20-abx125375 |
Abbexa |
-
EUR 710.40
-
EUR 1028.40
-
EUR 493.20
|
|
|
Actin-pan Antibody |
20-abx013019 |
Abbexa |
-
EUR 376.80
-
EUR 117.60
-
EUR 477.60
-
EUR 594.00
|
- 100 ug
- 10 ug
- 200 ug
- 300 µg
|
|
Synuclein-pan Antibody |
20-abx013202 |
Abbexa |
-
EUR 376.80
-
EUR 117.60
-
EUR 477.60
-
EUR 594.00
|
- 100 ug
- 10 ug
- 200 ug
- 300 µg
|
|
Pan pLDH Antibody |
abx023860-1mg |
Abbexa |
1 mg |
EUR 410.4 |
|
Pan pLDH Antibody |
abx023861-1mg |
Abbexa |
1 mg |
EUR 410.4 |
|
Pan SUMO Antibody |
abx027085-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Pan SUMO Antibody |
abx027085-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Pan SUMO Antibody |
abx027140-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Pan SUMO Antibody |
abx027140-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Pan myristoylation Antibody |
20-abx322788 |
Abbexa |
|
|
|
Pan myristoylation Antibody |
20-abx322789 |
Abbexa |
|
|
|
pan Cadherin Antibody |
abx217630-100ug |
Abbexa |
100 ug |
EUR 526.8 |
|
Pan Ubiquitin Antibody |
abx415347-50ug |
Abbexa |
50 ug |
EUR 678 |
|
pan ADH Antibody |
abx433090-200ul |
Abbexa |
200 ul |
EUR 460.8 |
|
SHANK (pan) Antibody |
abx440129-100ug |
Abbexa |
100 ug |
EUR 693.6 |
|
SAPAP (pan) Antibody |
abx440140-100ug |
Abbexa |
100 ug |
EUR 693.6 |
|
SHANK (pan) Antibody |
abx442377-100ug |
Abbexa |
100 ug |
EUR 693.6 |
|
SAPAP (pan) Antibody |
abx442388-100ug |
Abbexa |
100 ug |
EUR 693.6 |
|
Ankyrin (pan) Antibody |
abx442494-100ug |
Abbexa |
100 ug |
EUR 693.6 |
|
SHANK (pan) Antibody |
abx442658-100ug |
Abbexa |
100 ug |
EUR 693.6 |
|
SAPAP (pan) Antibody |
abx442669-100ug |
Abbexa |
100 ug |
EUR 693.6 |
|
Ankyrin (pan) Antibody |
abx442775-100ug |
Abbexa |
100 ug |
EUR 693.6 |
|
SHANK (pan) Antibody |
abx442938-100ug |
Abbexa |
100 ug |
EUR 693.6 |
|
SAPAP (pan) Antibody |
abx442949-100ug |
Abbexa |
100 ug |
EUR 693.6 |
|
Ankyrin (pan) Antibody |
abx443055-100ug |
Abbexa |
100 ug |
EUR 693.6 |
|
SHANK (pan) Antibody |
abx443218-100ug |
Abbexa |
100 ug |
EUR 678 |
|
SAPAP (pan) Antibody |
abx443229-100ug |
Abbexa |
100 ug |
EUR 678 |
|
Ankyrin (pan) Antibody |
abx443335-100ug |
Abbexa |
100 ug |
EUR 678 |
|
SHANK (pan) Antibody |
abx443499-100ug |
Abbexa |
100 ug |
EUR 678 |
|
SAPAP (pan) Antibody |
abx443510-100ug |
Abbexa |
100 ug |
EUR 678 |
|
Ankyrin (pan) Antibody |
abx443616-100ug |
Abbexa |
100 ug |
EUR 678 |
|
SHANK (pan) Antibody |
abx443780-100ug |
Abbexa |
100 ug |
EUR 710.4 |
|
The availability of the proposed pipeline will enable environment friendly, correct and reproducible RGC quantification required for assessing the loss of life/survival of RGCs in illness fashions.
Key phrases: Cell segmentation; Fluorescent microscopy; Homomorphic filter; On-line parameter tuning; Retinal ganglion cell (RGC)
Liriopesides B induces apoptosis and cell cycle arrest in human non‑small cell lung most cancers cells
Dedicator of cytokinesis 2 (DOCK2) is important for the B cell differentiation, BCR signaling and humoral immune response.
Nevertheless, the function of DOCK2 within the reminiscence response of B cell is unknown.
Through the use of two DOCK2 poor sufferers, we discovered that the reminiscence B cells have been decreased and the early activation of DOCK2 poor reminiscence B cells was abolished to the diploma of naïve B cells as a result of decreased expression of CD19 and CD21 mechanistically.
Apparently the expression of LEF-1, a adverse regulator of CD21, was elevated in DOCK2 poor B cells.
This was linked to the elevated expression of HIF-1α and cell metabolism, which in flip affected the ER construction.
Lastly, the discount of reminiscence B cells in DOCK2 sufferers was as a result of elevated apoptosis, which may be associated with the elevated metabolism.
Key phrases: B cell; DOCK2; LEF-1
B cell zone reticular cell microenvironments form CXCL13 gradient formation
By way of the formation of focus gradients, morphogens drive graded responses to extracellular alerts, thereby fine-tuning cell behaviors in advanced tissues.
Right here we present that the chemokine CXCL13 varieties each soluble and immobilized gradients.
Particularly, CXCL13+follicular reticular cells kind a small-world community of steerage constructions, with pc simulations and optimization evaluation predicting that immobilized gradients created by this community promote B cell trafficking.
In step with this prediction, imaging evaluation present that CXCL13 binds to extracellular matrix parts in situ, constraining its diffusion. CXCL13 solubilization requires the protease cathepsin B that cleaves CXCL13 right into a secure product.
Mice missing cathepsin B show aberrant follicular structure, a phenotype related to efficient B cell homing to however not inside lymph nodes.
Our knowledge thus recommend that reticular cells of the B cell zone generate microenvironments that form each immobilized and soluble CXCL13 gradients
Rat Immunoglobulin M (IgM) ELISA Kit |
DLR-IgM-Ra-48T |
DL Develop |
48T |
EUR 511.2 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Immunoglobulin M (IgM) in samples from serum or plasma. |
Rat Immunoglobulin M (IgM) ELISA Kit |
DLR-IgM-Ra-96T |
DL Develop |
96T |
EUR 658.8 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Immunoglobulin M (IgM) in samples from serum or plasma. |
Rabbit Immunoglobulin M (IgM) ELISA Kit |
DLR-IgM-Rb-48T |
DL Develop |
48T |
EUR 609.6 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Immunoglobulin M (IgM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Rabbit Immunoglobulin M (IgM) ELISA Kit |
DLR-IgM-Rb-96T |
DL Develop |
96T |
EUR 793.2 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Immunoglobulin M (IgM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Bovine Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 567.6 |
Bovine Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 784.8 |
Chicken Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Ch-48Tests |
Reddot Biotech |
48 Tests |
EUR 524.4 |
Chicken Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Ch-96Tests |
Reddot Biotech |
96 Tests |
EUR 723.6 |
Human Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 493.2 |
Human Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 678 |
Mouse Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 502.8 |
Mouse Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 693.6 |
Rat Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 524.4 |
Rat Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 723.6 |
Rabbit Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Rb-48Tests |
Reddot Biotech |
48 Tests |
EUR 640.8 |
Rabbit Immunoglobulin M (IgM) ELISA Kit |
RDR-IgM-Rb-96Tests |
Reddot Biotech |
96 Tests |
EUR 890.4 |
Bovine Immunoglobulin M (IgM) ELISA Kit |
RD-IgM-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 543.6 |
Bovine Immunoglobulin M (IgM) ELISA Kit |
RD-IgM-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 750 |
Rat Immunoglobulin M (IgM) ELISA Kit |
RD-IgM-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 502.8 |
Rat Immunoglobulin M (IgM) ELISA Kit |
RD-IgM-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 692.4 |
Bovine Ig fraction |
31R-1001 |
Fitzgerald |
10 mg |
EUR 136.8 |
Description: Bovine Ig fraction control or blocking reagent |
Chicken Ig fraction |
31R-1004 |
Fitzgerald |
10 mg |
EUR 248.4 |
Description: Chicken Ig fraction control or blocking reagent |
Horse Ig fraction |
31R-1005 |
Fitzgerald |
10 mg |
EUR 136.8 |
Description: Horse Ig fraction control or blocking reagent |
Goat Ig fraction |
31R-1008 |
Fitzgerald |
10 mg |
EUR 136.8 |
Description: Goat Ig fraction control or blocking reagent |
Hamster Ig fraction |
31R-1009 |
Fitzgerald |
10 mg |
EUR 165.6 |
Description: Hamster Ig fraction control or blocking reagent |
Rabbit Ig fraction |
31R-1018 |
Fitzgerald |
10 mg |
EUR 136.8 |
Description: Rabbit Ig fraction control or blocking reagent |
Rat Ig fraction |
31R-1021 |
Fitzgerald |
10 mg |
EUR 138 |
Description: Rat Ig fraction control or blocking reagent |
Sheep Ig fraction |
31R-1023 |
Fitzgerald |
10 mg |
EUR 136.8 |
Description: Sheep Ig fraction control or blocking reagent |
Bovine Ig fraction |
31R-1025 |
Fitzgerald |
10 mg |
EUR 136.8 |
Description: Purified Bovine Ig fraction for use as a control or blocking reagent |
Chicken Ig fraction |
31R-1028 |
Fitzgerald |
10 mg |
EUR 248.4 |
Description: Purified Chicken Ig fraction for use as a control or blocking reagent |
Horse Ig fraction |
31R-1029 |
Fitzgerald |
10 mg |
EUR 136.8 |
Description: Purified Horse Ig fraction for use as a control or blocking reagent |
Goat Ig fraction |
31R-1032 |
Fitzgerald |
10 mg |
EUR 163.2 |
Description: Purified Goat Ig fraction for use as a control or blocking reagent |
Mouse Ig fraction |
31R-1040 |
Fitzgerald |
10 mg |
EUR 180 |
Description: Purified Mouse Ig fraction for use as a control or blocking reagent |
Rabbit Ig fraction |
31R-1042 |
Fitzgerald |
10 mg |
EUR 136.8 |
Description: Purified Rabbit Ig fraction for use as a control or blocking reagent |
Rat Ig fraction |
31R-1045 |
Fitzgerald |
10 mg |
EUR 138 |
Description: Purified Rat Ig fraction for use as a control or blocking reagent |
Sheep Ig fraction |
31R-1047 |
Fitzgerald |
10 mg |
EUR 136.8 |
Description: Purified Sheep Ig fraction for use as a control or blocking reagent |
BSA Fraction V |
30-AB75 |
Fitzgerald |
1 kg |
EUR 1290 |
Description: Standard Grade Bovine Serum Albumin, Cohn Fraction V, (99% pure) |
Mouse Anti-Meningococcal A/C/W/Y Oligosaccharides IgM (Combo) ELISA Kit, 96 tests, Quantitative |
600-889-IGM |
Alpha Diagnostics |
1 Kit |
EUR 1074 |
Human Anti-Meningococcal Group A/C/W/Y Oligosaccharides IgM (Combo) ELISA Kit, 96 tests, Quantitative |
600-884-IGM |
Alpha Diagnostics |
1 Kit |
EUR 1220.4 |
AFP antibody (Ig fraction) |
70R-14345 |
Fitzgerald |
1 ml |
EUR 166.8 |
Description: Goat polyclonal AFP antibody (Ig fraction) |
Guinea Pig Ig fraction |
31R-1007 |
Fitzgerald |
10 mg |
EUR 138 |
Description: Guinea Pig Ig fraction control or blocking reagent |
Guinea Pig Ig fraction |
31R-1031 |
Fitzgerald |
10 mg |
EUR 165.6 |
Description: Purified Guinea Pig Ig fraction for use as a control or blocking reagent |
FITC antibody (IgG fraction) |
20C-CR1056SP |
Fitzgerald |
1 ml |
EUR 289.2 |
Description: Rabbit polyclonal FITC antibody |
WSE-7422 EzSubcell Fraction |
2332338 |
Atto |
1unit |
EUR 477.6 |
Description: Fr act i onat i on of N ucl ei & M i t ochondr i a |
Albumin, Cohn fraction pH5.2 |
AR2440 |
Bio Basic |
50g |
EUR 180.06 |
|
Pyruvate Kinase antibody (IgG fraction) |
20C-CR7017GP |
Fitzgerald |
1 mL |
EUR 282 |
Description: Goat polyclonal Pyruvate Kinase antibody (IgG fraction) |
Pyruvate Kinase antibody (IgG fraction) |
20R-PG010 |
Fitzgerald |
1 ml |
EUR 282 |
Description: Goat polyclonal Pyruvate Kinase antibody (IgG fraction) |
Fraction V BSA 10% solution |
30R-AB022 |
Fitzgerald |
2x10ml |
EUR 232.8 |
Description: Bovine Serum Albumin, Crohn Fraction V 10% solution |
BSA Fraction V (pH 5.2) |
AK8245-0100 |
Akron Biotech |
100g |
Ask for price |
BSA Fraction V (pH 5.2) |
AK8245-0500 |
Akron Biotech |
500g |
Ask for price |
BSA Fraction V (pH 5.2) |
AK8245-1000 |
Akron Biotech |
1kg |
Ask for price |
BSA Fraction V (pH 7.0) |
AK8905-0100 |
Akron Biotech |
100g |
Ask for price |
BSA Fraction V (pH 7.0) |
AK8905-0500 |
Akron Biotech |
500g |
Ask for price |
BSA Fraction V (pH 7.0) |
AK8905-1000 |
Akron Biotech |
1kg |
Ask for price |
BSA Cohn Fraction V (Microbiological Grade) |
30-AB80 |
Fitzgerald |
1 kg |
EUR 1072.8 |
Description: Microbiological Grade Bovine Serum Albumin, Cohn Fraction V, (99% pure) |
Fraction V Microbiological grade BSA powder |
BAC61-0050 |
Equitech |
50gm |
EUR 365.04 |
|
Fraction V Microbiological grade BSA powder |
BAC61-0100 |
Equitech |
100gm |
EUR 447.72 |
|
Fraction V Microbiological grade BSA powder |
BAC61-0500 |
Equitech |
500gm |
EUR 585 |
|
Fraction V Microbiological grade BSA powder |
BAC61-1000 |
Equitech |
1KG |
Ask for price |
|
Fraction V Microbiological grade BSA powder |
BAC61-10000 |
Equitech |
10KG |
Ask for price |
|
Fraction V BSA powder, pH 7 |
BAC62-0050 |
Equitech |
50gm |
EUR 407.16 |
|
Fraction V BSA powder, pH 7 |
BAC62-0100 |
Equitech |
100gm |
EUR 489.84 |
|
Fraction V BSA powder, pH 7 |
BAC62-0500 |
Equitech |
500gm |
EUR 702 |
|
Fraction V BSA powder, pH 7 |
BAC62-1000 |
Equitech |
1KG |
EUR 1014 |
|
Fraction V BSA powder, pH 7 |
BAC62-10000 |
Equitech |
10KG |
Ask for price |
|
Fraction V BSA powder, pH 5.2 |
BAC63-0050 |
Equitech |
50gm |
EUR 407.16 |
|
Fraction V BSA powder, pH 5.2 |
BAC63-0100 |
Equitech |
100gm |
EUR 489.84 |
|
Fraction V BSA powder, pH 5.2 |
BAC63-0500 |
Equitech |
500gm |
EUR 702 |
|