Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation
December 10, 2020

Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation

By Charles

Immune evasion is a vital most cancers hallmark and the understanding of its mechanisms has generated profitable therapeutic approaches. Induction of immunogenic cell demise (ICD) is anticipated to draw immune cell populations that promote innate and adaptive immune responses. Right here, we current a important advance for our adenovirus-mediated gene remedy method, the place the mixed p14ARF and human interferon-β (IFNβ) gene switch to human melanoma cells led to oncolysis, ICD and subsequent activation of immune cells. Our outcomes point out that IFNβ alone or together with p14ARF was capable of induce large cell demise within the human melanoma cell line SK-MEL-147, although caspase 3/7 activation was not important.

In situ gene remedy of s.c. SK-MEL-147 tumors in Nod-Scid mice revealed inhibition of tumor development and elevated survival in response to IFNβ alone or together with p14ARF. Emission of important markers of ICD (exposition of calreticulin, secretion of ATP and IFNβ) was stronger when cells had been handled with mixed p14ARF and IFNβ gene switch. Co-culture of beforehand transduced SK-MEL-147 cells with monocyte-derived dendritic cells (Mo-DCs) derived from wholesome donors resulted in elevated ranges of activation markers HLA-DR, CD80, and CD86.

Activated Mo-DCs had been capable of prime autologous and allogeneic T cells, leading to elevated secretion of IFNγ, TNF-α, and IL-10. Preliminary knowledge confirmed that T cells primed by Mo-DCs activated with p14ARF+IFNβ-transduced SK-MEL-147 cells had been capable of induce the lack of viability of contemporary non-transduced SK-MEL-147 cells, suggesting the induction of a particular cytotoxic inhabitants that acknowledged and killed SK-MEL-147 cells. Collectively, our outcomes point out that p14ARF and IFNβ delivered by our adenoviral system induced oncolysis in human melanoma cells accompanied by adaptive immune response activation and regulation.

 

LT-Okay63 Enhances B Cell Activation and Survival Elements in Neonatal Mice That Interprets Into Lengthy-Lived Humoral Immunity

Adjuvants improve magnitude and length of immune responses induced by vaccines. On this research we assessed in neonatal mice if and the way the adjuvant LT-Okay63 given with a pneumococcal conjugate vaccine, Pnc1-TT, may have an effect on the expression of tumor necrosis issue receptor (TNF-R) superfamily members, identified to be concerned within the initiation and upkeep of antibody responses; B cell activating issue receptor (BAFF-R) and B cell maturation antigen (BCMA) and their ligands, BAFF, and a proliferation inducing ligand (APRIL). Initially we assessed the maturation standing of various B cell populations and their expression of BAFF-R and BCMA. Neonatal mice had dramatically fewer B cells than grownup mice and the composition of various subsets throughout the B cell pool differed tremendously.
Proportionally newly fashioned B cells had been most considerable, however they’d diminished BAFF-R expression which may clarify low proportions of marginal zone and follicular B cells noticed. Restricted BCMA expression was additionally detected in neonatal pre-plasmablasts/plasmablasts. LT-Okay63 enhanced vaccine-induced BAFF-R expression in splenic marginal zone, follicular and newly fashioned B cells, resulting in elevated plasmablast/plasma cells, and their enhanced expression of BCMA in spleen and bone marrow. Moreover, the induction of BAFF and APRIL expression occurred early in neonatal mice immunized with Pnc1-TT both with or with out LT-Okay63. Nonetheless, BAFF+ and APRIL+ cells in spleens had been maintained at a better degree in mice that obtained the adjuvant. Moreover, the early improve of APRIL+ cells in bone marrow was extra profound in mice immunized with vaccine and adjuvant.
Lastly, we assessed, for the primary time in neonatal mice, accent cells of the plasma cell area of interest in bone marrow and their secretion of APRIL. We discovered that LT-Okay63 enhanced the frequency and APRIL expression of eosinophils, macrophages, and megakaryocytes, which probably contributed to plasma cell survival, regardless that APRIL+ cells confirmed a quick decline. All this was related to enhanced, sustained vaccine-specific antibody-secreting cells in bone marrow and persisting vaccine-specific serum antibodies. Our research sheds mild on the mechanisms behind the adjuvanticity of LT-Okay63 and identifies molecular pathways that must be triggered by vaccine adjuvants to induce sustained humoral immunity in formative years.
 Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation

Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation

Leishmania amazonensis Promastigotes or Extracellular Vesicles Modulate B-1 Cell Activation and Differentiation

B-1 cells are thought-about an innate-like B cell inhabitants that participates in efficient innate and adaptive responses to pathogens. B-1 cells produce immunoglobulins, cytokines, chemokines, migrate to inflammatory websites, and differentiate into mononuclear phagocyte-like cells. Murine B-1 cells phagocytosed Leishmania in vitro and in vivo and take part in immunity in opposition to Leishmania. Our group confirmed that B-1 cells or their extracellular vesicles (EVs) led to a resistance to experimental an infection by L. amazonensis. Nonetheless, the B-1 cells’ responses to Leishmania or EVs remoted from parasites are nonetheless poorly characterised. Learning the activation and differentiation of B-1 cells in vivo can contribute to a greater understanding of how these cells take part in immunity to L. amazonensis.
Thus, we evaluated the expression of myeloid (M-csfr, G-csfr, Spi-1) and lymphoid (EBF, E2A, IL-7R) lineage dedication components, Toll-like receptors (TLRs), activation cell floor markers, nitric oxide (NO) and reactive oxygen species (ROS) manufacturing in murine peritoneal B-1 cells collected after 24 or 48 h post-infection with Leishmania (Leishmania) amazonensis promastigotes or EVs launched by the parasites. Our outcomes demonstrated that L. amazonensis an infection didn’t stimulate the expression of CD40, CD80, CD86, F4/80, and MHC II in B-1 cells, however a major lower within the manufacturing of NO and ROS was noticed.

Casein Kappa (CSN3) Antibody

20-abx171599
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  • 1 mg
  • 200 ug

Casein Kappa (CSN3) Antibody

20-abx171600
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  • 1 mg
  • 200 ug

Casein Kappa (CSN3) Antibody

abx129181-100l 100 µl
EUR 275

Casein Kappa (CSN3) Antibody

abx129181-1ml 1 ml
EUR 750

Casein Kappa (CSN3) Antibody

abx129181-200l 200 µl
EUR 337.5

Casein Kappa (CSN3) Antibody

abx130922-100l 100 µl
EUR 887.5

Casein Kappa (CSN3) Antibody

abx130922-20l 20 µl
EUR 300

Casein Kappa (CSN3) Antibody

abx130922-50l 50 µl
EUR 387.5

Rabbit anti Casein (kappa) Polyclonal Antibody

TA354541 100 µg Ask for price

Kappa Casein (CSN3) Antibody

abx232019-100ug 100 ug
EUR 577.2

Kappa Casein (CSN3) Antibody

abx232019-100g 100 µg
EUR 350

Recombinant Casein Kappa (CSN3)

RPU53251-100ug 100ug
EUR 542.3

Recombinant Casein Kappa (CSN3)

RPU53251-1mg 1mg
EUR 2402.4

Recombinant Casein Kappa (CSN3)

RPU53251-50ug 50ug
EUR 435.6

Recombinant Casein Kappa (CSN3)

RPU54682-100ug 100ug
EUR 640.2

Recombinant Casein Kappa (CSN3)

RPU54682-1mg 1mg
EUR 2839.2

Recombinant Casein Kappa (CSN3)

RPU54682-50ug 50ug
EUR 514.8

Recombinant Casein Kappa (CSN3)

RPJ331Bo01 10ug
EUR 208

Recombinant Casein Kappa (CSN3)

4-RPJ331Bo01
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  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Bovine Casein Kappa expressed in: E.coli

Recombinant Casein Kappa (CSN3)

RPJ331Hu01 10ug
EUR 176

Recombinant Casein Kappa (CSN3)

4-RPJ331Hu01
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  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Casein Kappa expressed in: E.coli

Casein Kappa (CSN3) Polyclonal Antibody

CAU29057-100ul 100ul
EUR 279.4

Casein Kappa (CSN3) Polyclonal Antibody

CAU29057-200ul 200ul
EUR 349.4

Casein Kappa (CSN3) Polyclonal Antibody

CAU21739-100ul 100ul
EUR 235.2

Casein Kappa (CSN3) Polyclonal Antibody

CAU21739-200ul 200ul
EUR 294

Human Casein Kappa ELISA Kit

IHUCSN3KT each
EUR 733
Description: Human Casein Kappa ELISA Kit

Cow Casein Kappa (CSN3) Protein

20-abx650845
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Bovine Casein Kappa ELISA Kit

IBOCSN3KT each
EUR 866
Description: Bovine Casein Kappa ELISA Kit

Cow Casein Kappa (CSN3) Protein

abx650845-5mg 5 mg
EUR 662.5

Human Casein Kappa (CSN3) Protein

20-abx167038
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  • 100 ug
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  • 1 mg
  • 200 ug
  • 50 ug

Human Casein Kappa (CSN3) Protein

abx167038-100g 100 µg
EUR 575

Human Casein Kappa (CSN3) Protein

abx167038-10g 10 µg
EUR 225

Human Casein Kappa (CSN3) Protein

abx167038-50g 50 µg
EUR 400

Polyclonal Antibody to Casein Kappa (CSN3)

PAJ331Bo01 100ul
EUR 291

Polyclonal Antibody to Casein Kappa (CSN3)

PAJ331Hu01 100ul
EUR 245

Cow Casein kappa (CSN3) CLIA Kit

20-abx495684
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Cow Casein kappa (CSN3) ELISA Kit

20-abx150084
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

ELISA Kit for Casein Kappa (CSN3)

SEJ331Bo 96Т
EUR 840

ELISA Kit for Casein Kappa (CSN3)

SEJ331Hu 96Т
EUR 700

Casein Kappa (CSN3) Polyclonal Antibody (Human)

4-PAJ331Hu01
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  • 100ul
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Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3)

Human Casein kappa (CSN3) CLIA Kit

20-abx495685
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  • 10 × 96 tests
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  • 96 tests

Casein Kappa (CSN3) Polyclonal Antibody (Bovine)

4-PAJ331Bo01
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
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Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3)

Human Casein Kappa (CSN3)ELISA Kit

201-12-2883 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

DLR-CSN3-Hu 96T
EUR 463
Description: serum, plasma or other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

DLR-CSN3-Hu-48T 48T
EUR 620.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Casein Kappa (CSN3) in samples from serum, plasma or other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

DLR-CSN3-Hu-96T 96T
EUR 807.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Casein Kappa (CSN3) in samples from serum, plasma or other biological fluids.

Human CSN3(Casein Kappa) ELISA Kit

ELK4819-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CSN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CSN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CSN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CSN3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human CSN3(Casein Kappa) ELISA Kit

ELK4819-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CSN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CSN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CSN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CSN3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Casein Kappa (CSN3) ELISA Kit

EKN44092-48T 48T
EUR 378.7

Human Casein Kappa (CSN3) ELISA Kit

EKN44092-5x96T 5x96T
EUR 2569.75

Human Casein Kappa (CSN3) ELISA Kit

EKN44092-96T 96T
EUR 541

Human Casein Kappa (CSN3) ELISA Kit

EKL56543-5x96T 5x96T
EUR 3793.35

Human Casein Kappa (CSN3) ELISA Kit

EKL56543-96T 96T
EUR 798.6

Human Casein Kappa (CSN3) ELISA Kit

EKU02981-48T 48T
EUR 555.66

Human Casein Kappa (CSN3) ELISA Kit

EKU02981-5x96T 5x96T
EUR 3770.55

Human Casein Kappa (CSN3) ELISA Kit

EKU02981-96T 96T
EUR 793.8

Human Casein Kappa (CSN3) ELISA Kit

DL-CSN3-Hu 96T
EUR 441
Description: serum, plasma or other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

EK13477 96Т
EUR 768

Human Casein kappa (CSN3) ELISA Kit

20-abx150942
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-Hu-48T 48T
EUR 475.34
Description: serum, plasma and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-Hu-48Tests 48 Tests
EUR 652.8

Human Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-Hu-96T 96T
EUR 679.04
Description: serum, plasma and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-Hu-96Tests 96 Tests
EUR 907.2

Human Casein Kappa (CSN3) ELISA Kit

RD-CSN3-Hu-48T 48T
EUR 452.7
Description: serum, plasma and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

RD-CSN3-Hu-48Tests 48 Tests
EUR 625.2

Human Casein Kappa (CSN3) ELISA Kit

RD-CSN3-Hu-96T 96T
EUR 646.7
Description: serum, plasma and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

RD-CSN3-Hu-96Tests 96 Tests
EUR 867.6

Human Casein Kappa ELISA Kit (CSN3)

RK01196 96 Tests
EUR 625.2

Human Casein Kappa(CSN3)ELISA Kit

QY-E02404 96T
EUR 433.2

Human Casein Kappa (CSN3) ELISA Kit

SEJ331Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Casein Kappa (CSN3) in Breast milk and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

SEJ331Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Casein Kappa (CSN3) in Breast milk and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

SEJ331Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Casein Kappa (CSN3) in Breast milk and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

SEJ331Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Casein Kappa (CSN3) in Breast milk and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

4-SEJ331Hu
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  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Casein Kappa (CSN3) in samples from Breast milk and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Casein Kappa (CSN3)ELISA Kit

YLA3486HU-48T 48T Ask for price

Human Casein Kappa (CSN3)ELISA Kit

YLA3486HU-96T 96T Ask for price

Bovine Casein Kappa (CSN3) ELISA Kit

DLR-CSN3-b 96T
EUR 547
Description: milk.

Bovine Casein Kappa (CSN3) ELISA Kit

DLR-CSN3-b-48T 48T
EUR 710.4
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Casein Kappa (CSN3) in samples from milk.

Bovine Casein Kappa (CSN3) ELISA Kit

DLR-CSN3-b-96T 96T
EUR 932.4
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Casein Kappa (CSN3) in samples from milk.

Cattle CSN3(Casein Kappa) ELISA Kit

ELK8007-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle CSN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle CSN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle CSN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle CSN3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Cattle CSN3(Casein Kappa) ELISA Kit

ELK8007-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle CSN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle CSN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle CSN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle CSN3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Bovine Casein Kappa (CSN3) ELISA Kit

EKN44091-48T 48T
EUR 454.72

Bovine Casein Kappa (CSN3) ELISA Kit

EKN44091-5x96T 5x96T
EUR 3085.6

Bovine Casein Kappa (CSN3) ELISA Kit

EKN44091-96T 96T
EUR 649.6

Bovine Casein Kappa (CSN3) ELISA Kit

EKU02980-48T 48T
EUR 666.82

Bovine Casein Kappa (CSN3) ELISA Kit

EKU02980-5x96T 5x96T
EUR 4524.85

Bovine Casein Kappa (CSN3) ELISA Kit

EKU02980-96T 96T
EUR 952.6

Bovine Casein Kappa (CSN3) ELISA Kit

DL-CSN3-b 96T
EUR 521
Description: milk.

Bovine Casein Kappa (CSN3) ELISA Kit

EK13476 96Т
EUR 768

Bovine Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-b-48T 48T
EUR 561.02
Description: milk.

Bovine Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-b-48Tests 48 Tests
EUR 759.6

Bovine Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-b-96T 96T
EUR 801.47
Description: milk.

Bovine Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-b-96Tests 96 Tests
EUR 1060.8

Bovine Casein Kappa (CSN3) ELISA Kit

RD-CSN3-b-48T 48T
EUR 534.3
Description: milk.

Bovine Casein Kappa (CSN3) ELISA Kit

RD-CSN3-b-48Tests 48 Tests
EUR 727.2

Bovine Casein Kappa (CSN3) ELISA Kit

RD-CSN3-b-96T 96T
EUR 763.3
Description: milk.

Bovine Casein Kappa (CSN3) ELISA Kit

RD-CSN3-b-96Tests 96 Tests
EUR 1012.8

Bovine Casein Kappa ELISA Kit (CSN3)

RK00450 96 Tests
EUR 687.6

Bovine Casein Kappa (CSN3) ELISA Kit

SL0215Bo - Ask for price

Cattle Casein Kappa (CSN3) ELISA Kit

SEJ331Bo-10x96wellstestplate 10x96-wells test plate
EUR 6777.36
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Casein Kappa (CSN3) in milk.

Cattle Casein Kappa (CSN3) ELISA Kit

SEJ331Bo-1x48wellstestplate 1x48-wells test plate
EUR 663.31
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Casein Kappa (CSN3) in milk.

Cattle Casein Kappa (CSN3) ELISA Kit

SEJ331Bo-1x96wellstestplate 1x96-wells test plate
EUR 896.16
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Casein Kappa (CSN3) in milk.

Cattle Casein Kappa (CSN3) ELISA Kit

SEJ331Bo-5x96wellstestplate 5x96-wells test plate
EUR 3672.72
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Casein Kappa (CSN3) in milk.

Cattle Casein Kappa (CSN3) ELISA Kit

4-SEJ331Bo
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  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Cattle Casein Kappa (CSN3) in samples from milk with no significant corss-reactivity with analogues from other species.

CSN3 (untagged)-Human casein kappa (CSN3)

SC126241 10 µg Ask for price

Human Casein Kappa, CSN3 GENLISA ELISA

KBH2882 1 x 96 wells
EUR 286

Casein Kappa (CSN3) Polyclonal Antibody (Human), PE

4-PAJ331Hu01-PE
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with PE.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), PE

4-PAJ331Bo01-PE
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with PE.

Casein Kappa (CSN3) Polyclonal Antibody (Human), APC

4-PAJ331Hu01-APC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with APC.

Casein Kappa (CSN3) Polyclonal Antibody (Human), Cy3

4-PAJ331Hu01-Cy3
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with Cy3.

Casein Kappa (CSN3) Polyclonal Antibody (Human), HRP

4-PAJ331Hu01-HRP
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with HRP.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), APC

4-PAJ331Bo01-APC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with APC.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), Cy3

4-PAJ331Bo01-Cy3
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with Cy3.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), HRP

4-PAJ331Bo01-HRP
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with HRP.

Casein Kappa (CSN3) Polyclonal Antibody (Human), FITC

4-PAJ331Hu01-FITC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with FITC.

ELISA kit for Human CSN3 (Casein Kappa)

ELK4819 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Casein Kappa from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), FITC

4-PAJ331Bo01-FITC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with FITC.

Rat Kappa casein(CSN3) ELISA kit

E01A14264 96T
EUR 700
Description: ELISA

Rat Kappa casein(CSN3) ELISA kit

E02K0085-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Kappa casein(CSN3) ELISA kit

E02K0085-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Kappa casein(CSN3) ELISA kit

E02K0085-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
The an infection induced a considerably larger arginase expression in B-1 cells, however the stimulation with EVs led to a lower on this gene expression. TLR-2 and TLR-6 had considerably larger expression in B-1 cells from mice intraperitoneally stimulated with the parasite. The TLR-9 expression was larger in animals contaminated or stimulated for 48 h with EVs. Apparently, in B-1 cells the stimulus with L. amazonensis led to a considerable improve within the expression of myeloid restricted transcription components. Thus, our research means that the parasites or EVs in another way modulated the activation and differentiation of B-1 cells.