Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation
December 10, 2020

Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation

By Charles

Immune evasion is a vital most cancers hallmark and the understanding of its mechanisms has generated profitable therapeutic approaches. Induction of immunogenic cell demise (ICD) is anticipated to draw immune cell populations that promote innate and adaptive immune responses. Right here, we current a important advance for our adenovirus-mediated gene remedy method, the place the mixed p14ARF and human interferon-β (IFNβ) gene switch to human melanoma cells led to oncolysis, ICD and subsequent activation of immune cells. Our outcomes point out that IFNβ alone or together with p14ARF was capable of induce large cell demise within the human melanoma cell line SK-MEL-147, although caspase 3/7 activation was not important.

In situ gene remedy of s.c. SK-MEL-147 tumors in Nod-Scid mice revealed inhibition of tumor development and elevated survival in response to IFNβ alone or together with p14ARF. Emission of important markers of ICD (exposition of calreticulin, secretion of ATP and IFNβ) was stronger when cells had been handled with mixed p14ARF and IFNβ gene switch. Co-culture of beforehand transduced SK-MEL-147 cells with monocyte-derived dendritic cells (Mo-DCs) derived from wholesome donors resulted in elevated ranges of activation markers HLA-DR, CD80, and CD86.

Activated Mo-DCs had been capable of prime autologous and allogeneic T cells, leading to elevated secretion of IFNγ, TNF-α, and IL-10. Preliminary knowledge confirmed that T cells primed by Mo-DCs activated with p14ARF+IFNβ-transduced SK-MEL-147 cells had been capable of induce the lack of viability of contemporary non-transduced SK-MEL-147 cells, suggesting the induction of a particular cytotoxic inhabitants that acknowledged and killed SK-MEL-147 cells. Collectively, our outcomes point out that p14ARF and IFNβ delivered by our adenoviral system induced oncolysis in human melanoma cells accompanied by adaptive immune response activation and regulation.

 

LT-Okay63 Enhances B Cell Activation and Survival Elements in Neonatal Mice That Interprets Into Lengthy-Lived Humoral Immunity

Adjuvants improve magnitude and length of immune responses induced by vaccines. On this research we assessed in neonatal mice if and the way the adjuvant LT-Okay63 given with a pneumococcal conjugate vaccine, Pnc1-TT, may have an effect on the expression of tumor necrosis issue receptor (TNF-R) superfamily members, identified to be concerned within the initiation and upkeep of antibody responses; B cell activating issue receptor (BAFF-R) and B cell maturation antigen (BCMA) and their ligands, BAFF, and a proliferation inducing ligand (APRIL). Initially we assessed the maturation standing of various B cell populations and their expression of BAFF-R and BCMA. Neonatal mice had dramatically fewer B cells than grownup mice and the composition of various subsets throughout the B cell pool differed tremendously.
Proportionally newly fashioned B cells had been most considerable, however they’d diminished BAFF-R expression which may clarify low proportions of marginal zone and follicular B cells noticed. Restricted BCMA expression was additionally detected in neonatal pre-plasmablasts/plasmablasts. LT-Okay63 enhanced vaccine-induced BAFF-R expression in splenic marginal zone, follicular and newly fashioned B cells, resulting in elevated plasmablast/plasma cells, and their enhanced expression of BCMA in spleen and bone marrow. Moreover, the induction of BAFF and APRIL expression occurred early in neonatal mice immunized with Pnc1-TT both with or with out LT-Okay63. Nonetheless, BAFF+ and APRIL+ cells in spleens had been maintained at a better degree in mice that obtained the adjuvant. Moreover, the early improve of APRIL+ cells in bone marrow was extra profound in mice immunized with vaccine and adjuvant.
Lastly, we assessed, for the primary time in neonatal mice, accent cells of the plasma cell area of interest in bone marrow and their secretion of APRIL. We discovered that LT-Okay63 enhanced the frequency and APRIL expression of eosinophils, macrophages, and megakaryocytes, which probably contributed to plasma cell survival, regardless that APRIL+ cells confirmed a quick decline. All this was related to enhanced, sustained vaccine-specific antibody-secreting cells in bone marrow and persisting vaccine-specific serum antibodies. Our research sheds mild on the mechanisms behind the adjuvanticity of LT-Okay63 and identifies molecular pathways that must be triggered by vaccine adjuvants to induce sustained humoral immunity in formative years.
 Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation

Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation

Leishmania amazonensis Promastigotes or Extracellular Vesicles Modulate B-1 Cell Activation and Differentiation

B-1 cells are thought-about an innate-like B cell inhabitants that participates in efficient innate and adaptive responses to pathogens. B-1 cells produce immunoglobulins, cytokines, chemokines, migrate to inflammatory websites, and differentiate into mononuclear phagocyte-like cells. Murine B-1 cells phagocytosed Leishmania in vitro and in vivo and take part in immunity in opposition to Leishmania. Our group confirmed that B-1 cells or their extracellular vesicles (EVs) led to a resistance to experimental an infection by L. amazonensis. Nonetheless, the B-1 cells’ responses to Leishmania or EVs remoted from parasites are nonetheless poorly characterised. Learning the activation and differentiation of B-1 cells in vivo can contribute to a greater understanding of how these cells take part in immunity to L. amazonensis.
Thus, we evaluated the expression of myeloid (M-csfr, G-csfr, Spi-1) and lymphoid (EBF, E2A, IL-7R) lineage dedication components, Toll-like receptors (TLRs), activation cell floor markers, nitric oxide (NO) and reactive oxygen species (ROS) manufacturing in murine peritoneal B-1 cells collected after 24 or 48 h post-infection with Leishmania (Leishmania) amazonensis promastigotes or EVs launched by the parasites. Our outcomes demonstrated that L. amazonensis an infection didn’t stimulate the expression of CD40, CD80, CD86, F4/80, and MHC II in B-1 cells, however a major lower within the manufacturing of NO and ROS was noticed.

Casein Kappa (CSN3) Antibody

20-abx129181
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Casein Kappa (CSN3) Antibody

20-abx130922
  • EUR 481.00
  • EUR 133.00
  • EUR 1400.00
  • EUR 662.00
  • EUR 356.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Casein Kappa (CSN3) Antibody

20-abx171599
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug

Casein Kappa (CSN3) Antibody

20-abx171600
  • EUR 982.00
  • EUR 495.00
  • 1 mg
  • 200 ug

Cow Casein Kappa (CSN3) Protein

20-abx650845
  • EUR 787.00
  • EUR 300.00
  • EUR 2486.00
  • EUR 940.00
  • EUR 551.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Casein Kappa (CSN3) Protein

20-abx167038
  • EUR 690.00
  • EUR 286.00
  • EUR 2124.00
  • EUR 815.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Casein Kappa (CSN3) Polyclonal Antibody (Bovine)

4-PAJ331Bo01
  • EUR 274.00
  • EUR 2945.00
  • EUR 724.00
  • EUR 349.00
  • EUR 226.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3)

Casein Kappa (CSN3) Polyclonal Antibody (Human)

4-PAJ331Hu01
  • EUR 247.00
  • EUR 2510.00
  • EUR 625.00
  • EUR 310.00
  • EUR 214.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3)

Cattle Casein Kappa (CSN3) ELISA Kit

SEJ331Bo-10x96wellstestplate 10x96-wells test plate
EUR 5647.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Casein Kappa (CSN3) in milk.

Cattle Casein Kappa (CSN3) ELISA Kit

SEJ331Bo-1x48wellstestplate 1x48-wells test plate
EUR 552.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Casein Kappa (CSN3) in milk.

Cattle Casein Kappa (CSN3) ELISA Kit

SEJ331Bo-1x96wellstestplate 1x96-wells test plate
EUR 746.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Casein Kappa (CSN3) in milk.

Cattle Casein Kappa (CSN3) ELISA Kit

SEJ331Bo-5x96wellstestplate 5x96-wells test plate
EUR 3060.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Casein Kappa (CSN3) in milk.

Cattle Casein Kappa (CSN3) ELISA Kit

4-SEJ331Bo
  • EUR 5698.00
  • EUR 3011.00
  • EUR 747.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Cattle Casein Kappa (CSN3) in samples from milk with no significant corss-reactivity with analogues from other species.

Human Casein Kappa (CSN3) ELISA Kit

SEJ331Hu-10x96wellstestplate 10x96-wells test plate
EUR 4731.5
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Casein Kappa (CSN3) in Breast milk and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

SEJ331Hu-1x48wellstestplate 1x48-wells test plate
EUR 477.3
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Casein Kappa (CSN3) in Breast milk and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

SEJ331Hu-1x96wellstestplate 1x96-wells test plate
EUR 639
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Casein Kappa (CSN3) in Breast milk and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

SEJ331Hu-5x96wellstestplate 5x96-wells test plate
EUR 2575.5
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Casein Kappa (CSN3) in Breast milk and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

4-SEJ331Hu
  • EUR 4782.00
  • EUR 2526.00
  • EUR 640.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Casein Kappa (CSN3) in samples from Breast milk and other biological fluids with no significant corss-reactivity with analogues from other species.

Bovine Kappa-casein(CSN3) ELISA kit

CSB-EL006064BO-24T 1 plate of 24 wells
EUR 165
Description: Quantitativecompetitive ELISA kit for measuring Bovine Kappa-casein (CSN3) in samples from breastmilk. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Bovine Kappa-casein(CSN3) ELISA kit

1-CSB-EL006064BO
  • EUR 900.00
  • EUR 5476.00
  • EUR 2900.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativecompetitive ELISA kit for measuring Bovine Kappa-casein(CSN3) in samples from breastmilk. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Kappa-casein(CSN3) ELISA kit

CSB-EL006064HU-24T 1 plate of 24 wells
EUR 165
Description: Quantitativecompetitive ELISA kit for measuring Human Kappa-casein (CSN3) in samples from serum, plasma, tissue homogenates, otherbiologicalfluids. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Kappa-casein(CSN3) ELISA kit

1-CSB-EL006064HU
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativecompetitive ELISA kit for measuring Human Kappa-casein(CSN3) in samples from serum, plasma, tissue homogenates, otherbiologicalfluids. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Bovine Casein Kappa (CSN3) ELISA Kit

RD-CSN3-b-48Tests 48 Tests
EUR 606

Bovine Casein Kappa (CSN3) ELISA Kit

RD-CSN3-b-96Tests 96 Tests
EUR 844

Human Casein Kappa (CSN3) ELISA Kit

RD-CSN3-Hu-48Tests 48 Tests
EUR 521

Human Casein Kappa (CSN3) ELISA Kit

RD-CSN3-Hu-96Tests 96 Tests
EUR 723

Bovine CSN3/ Kappa-casein ELISA Kit

E0074Bo 1 Kit
EUR 717

Rat Csn3/ Kappa-casein ELISA Kit

E0241Ra 1 Kit
EUR 646

Human CSN3/ Kappa-casein ELISA Kit

E0577Hu 1 Kit
EUR 605

Bovine Casein Kappa (CSN3) ELISA Kit

DLR-CSN3-b-48T 48T
EUR 592
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Casein Kappa (CSN3) in samples from milk.

Bovine Casein Kappa (CSN3) ELISA Kit

DLR-CSN3-b-96T 96T
EUR 777
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Casein Kappa (CSN3) in samples from milk.

Human Casein Kappa (CSN3) ELISA Kit

DLR-CSN3-Hu-48T 48T
EUR 517
Description: A sandwich quantitative ELISA assay kit for detection of Human Casein Kappa (CSN3) in samples from serum, plasma or other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

DLR-CSN3-Hu-96T 96T
EUR 673
Description: A sandwich quantitative ELISA assay kit for detection of Human Casein Kappa (CSN3) in samples from serum, plasma or other biological fluids.

Human Casein Kappa (CSN3)ELISA Kit

201-12-2883 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Rat Kappa Casein (CSN3) ELISA Kit

abx555992-96tests 96 tests
EUR 668

Mouse Kappa Casein (CSN3) ELISA Kit

abx556064-96tests 96 tests
EUR 668

Human Kappa Casein (CSN3) ELISA Kit

abx575410-96tests 96 tests
EUR 668

Cow Kappa Casein (CSN3) ELISA Kit

abx555452-96tests 96 tests
EUR 911

Pig Kappa Casein (CSN3) ELISA Kit

abx555666-96tests 96 tests
EUR 911

Cow Casein kappa (CSN3) CLIA Kit

20-abx495684
  • EUR 9242.00
  • EUR 4920.00
  • EUR 1130.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Casein kappa (CSN3) CLIA Kit

20-abx495685
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Casein kappa (CSN3) ELISA Kit

20-abx150942
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Cow Casein kappa (CSN3) ELISA Kit

20-abx150084
  • EUR 7911.00
  • EUR 4215.00
  • EUR 973.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Casein Kappa ELISA Kit (CSN3)

RK01196 96 Tests
EUR 521

Bovine Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-b-48Tests 48 Tests
EUR 633

Bovine Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-b-96Tests 96 Tests
EUR 884

Human Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-Hu-48Tests 48 Tests
EUR 544

Human Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-Hu-96Tests 96 Tests
EUR 756

Bovine Casein Kappa ELISA Kit (CSN3)

RK00450 96 Tests
EUR 573

Human Casein Kappa(CSN3)ELISA Kit

QY-E02404 96T
EUR 361

ELISA kit for Rat Kappa-casein

EK3314 96 tests
EUR 670
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Kappa-casein in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Bovine Kappa-casein

EK3315 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Bovine Kappa-casein in samples from serum, plasma, tissue homogenates and other biological fluids.

Bovine Kappa- casein, CSN3 ELISA KIT

ELI-23848b 96 Tests
EUR 928

Human Kappa- casein, CSN3 ELISA KIT

ELI-23849h 96 Tests
EUR 824

Porcine Kappa- casein, CSN3 ELISA KIT

ELI-23850p 96 Tests
EUR 928

Rat Kappa- casein, Csn3 ELISA KIT

ELI-33106r 96 Tests
EUR 886

Mouse Kappa- casein, Csn3 ELISA KIT

ELI-34476m 96 Tests
EUR 865

Monkey Kappa casein(CSN3) ELISA kit

E09K0085-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Monkey Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Kappa casein(CSN3) ELISA kit

E09K0085-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Monkey Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Kappa casein(CSN3) ELISA kit

E09K0085-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Monkey Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Kappa casein(CSN3) ELISA kit

E08K0085-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Canine Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Kappa casein(CSN3) ELISA kit

E08K0085-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Canine Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Kappa casein(CSN3) ELISA kit

E08K0085-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Canine Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Kappa casein(CSN3) ELISA kit

E01K0085-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Human Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Kappa casein(CSN3) ELISA kit

E01K0085-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Human Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Kappa casein(CSN3) ELISA kit

E01K0085-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Human Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Kappa casein(CSN3) ELISA kit

E03K0085-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Mouse Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Kappa casein(CSN3) ELISA kit

E03K0085-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Mouse Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Kappa casein(CSN3) ELISA kit

E03K0085-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Mouse Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Kappa casein(CSN3) ELISA kit

E02K0085-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Kappa casein(CSN3) ELISA kit

E02K0085-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Rat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Kappa casein(CSN3) ELISA kit

E02K0085-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Rat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Kappa casein(CSN3) ELISA kit

E07K0085-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Porcine Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Kappa casein(CSN3) ELISA kit

E07K0085-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Porcine Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Kappa casein(CSN3) ELISA kit

E07K0085-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Porcine Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Kappa casein(CSN3) ELISA kit

E06K0085-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Goat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Kappa casein(CSN3) ELISA kit

E06K0085-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Goat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Kappa casein(CSN3) ELISA kit

E06K0085-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Goat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Kappa casein(CSN3) ELISA kit

E04K0085-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rabbit Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Kappa casein(CSN3) ELISA kit

E04K0085-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Rabbit Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Kappa casein(CSN3) ELISA kit

E04K0085-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Rabbit Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Anti-Casein monoclonal antibody, clone CSD

HMABPY120D-100ug 100 ug
EUR 533

immunoglobulin Kappa (immunoglobulin kappa) Antibody

abx234295-100ug 100 ug
EUR 509

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), APC

4-PAJ331Bo01-APC
  • EUR 386.00
  • EUR 3869.00
  • EUR 1061.00
  • EUR 499.00
  • EUR 237.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with APC.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), Biotinylated

4-PAJ331Bo01-Biotin
  • EUR 342.00
  • EUR 2895.00
  • EUR 836.00
  • EUR 424.00
  • EUR 232.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with Biotin.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), Cy3

4-PAJ331Bo01-Cy3
  • EUR 474.00
  • EUR 5117.00
  • EUR 1373.00
  • EUR 624.00
  • EUR 275.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with Cy3.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), FITC

4-PAJ331Bo01-FITC
  • EUR 329.00
  • EUR 3115.00
  • EUR 868.00
  • EUR 419.00
  • EUR 210.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with FITC.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), HRP

4-PAJ331Bo01-HRP
  • EUR 351.00
  • EUR 3369.00
  • EUR 936.00
  • EUR 449.00
  • EUR 222.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with HRP.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), PE

4-PAJ331Bo01-PE
  • EUR 329.00
  • EUR 3115.00
  • EUR 868.00
  • EUR 419.00
  • EUR 210.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with PE.

Casein Kappa (CSN3) Polyclonal Antibody (Human), APC

4-PAJ331Hu01-APC
  • EUR 345.00
  • EUR 3275.00
  • EUR 912.00
  • EUR 440.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with APC.

Casein Kappa (CSN3) Polyclonal Antibody (Human), Biotinylated

4-PAJ331Hu01-Biotin
  • EUR 311.00
  • EUR 2460.00
  • EUR 727.00
  • EUR 381.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with Biotin.

Casein Kappa (CSN3) Polyclonal Antibody (Human), Cy3

4-PAJ331Hu01-Cy3
  • EUR 419.00
  • EUR 4325.00
  • EUR 1175.00
  • EUR 545.00
  • EUR 251.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with Cy3.

Casein Kappa (CSN3) Polyclonal Antibody (Human), FITC

4-PAJ331Hu01-FITC
  • EUR 296.00
  • EUR 2640.00
  • EUR 750.00
  • EUR 372.00
  • EUR 195.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with FITC.

Casein Kappa (CSN3) Polyclonal Antibody (Human), HRP

4-PAJ331Hu01-HRP
  • EUR 316.00
  • EUR 2855.00
  • EUR 807.00
  • EUR 398.00
  • EUR 206.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with HRP.

Casein Kappa (CSN3) Polyclonal Antibody (Human), PE

4-PAJ331Hu01-PE
  • EUR 296.00
  • EUR 2640.00
  • EUR 750.00
  • EUR 372.00
  • EUR 195.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with PE.

ELISA kit for Human CSN3 (Casein Kappa)

ELK4819 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Casein Kappa from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Human Kappa-casein (CSN3)

KTE62271-48T 48T
EUR 332
Description: Quantitative sandwich ELISA for measuring Human Kappa-casein (CSN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Kappa-casein (CSN3)

KTE62271-5platesof96wells 5 plates of 96 wells
EUR 2115
Description: Quantitative sandwich ELISA for measuring Human Kappa-casein (CSN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Kappa-casein (CSN3)

KTE62271-96T 96T
EUR 539
Description: Quantitative sandwich ELISA for measuring Human Kappa-casein (CSN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Cattle CSN3 (Casein Kappa)

ELK8007 1 plate of 96 wells
EUR 526
Description: A sandwich ELISA kit for detection of Casein Kappa from Cattle in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Guinea pig Kappa casein(CSN3) ELISA kit

E05K0085-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Guinea pig Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Kappa casein(CSN3) ELISA kit

E05K0085-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Guinea pig Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Kappa casein(CSN3) ELISA kit

E05K0085-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Guinea pig Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

anti-beta Casein

YF-PA23527 50 ul
EUR 334
Description: Mouse polyclonal to beta Casein

Anti-Kappa Light Chain Antibody Clone SPM508, Unconjugated-100ug

3514-MSM1X-P1 100ug
EUR 428

Anti-Kappa Light Chain Antibody Clone HP6053, Unconjugated-100ug

3514-MSM4-P1 100ug
EUR 428

Anti-Kappa Light Chain Antibody Clone KLC709, Unconjugated-100ug

3514-MSM6-P1 100ug
EUR 428

Anti-Kappa Light Chain Antibody Clone KLC1278, Unconjugated-100ug

3514-MSM8-P1 100ug
EUR 428

Recombinant Human Casein kinase I isoform epsilon Protein, His, E.coli-100ug

QP5880-ec-100ug 100ug
EUR 408

Recombinant Human Casein kinase I isoform epsilon Protein, His, Yeast-100ug

QP5880-ye-100ug 100ug
EUR 480

Recombinant Human Casein kinase I isoform epsilon Protein, GST, E.coli-100ug

QP8358-ec-100ug 100ug
EUR 408

Recombinant Human Casein kinase II subunit beta Protein, GST, E.coli-100ug

QP8381-ec-100ug 100ug
EUR 408

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), APC-Cy7

4-PAJ331Bo01-APC-Cy7
  • EUR 653.00
  • EUR 7618.00
  • EUR 2002.00
  • EUR 878.00
  • EUR 355.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with APC-Cy7.

Casein Kappa (CSN3) Polyclonal Antibody (Human), APC-Cy7

4-PAJ331Hu01-APC-Cy7
  • EUR 571.00
  • EUR 6430.00
  • EUR 1705.00
  • EUR 760.00
  • EUR 319.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with APC-Cy7.

Csn3 ELISA Kit| Rat Kappa-casein ELISA Kit

EF018435 96 Tests
EUR 689

Csn3 ELISA Kit| Mouse Kappa-casein ELISA Kit

EF014412 96 Tests
EUR 689

Casein Kinase 1 Delta (Casein Kinase 1 delta) Antibody

abx231279-100ug 100 ug
EUR 481

Casein Kinase 1 Epsilon (Casein Kinase 1 epsilon) Antibody

abx231280-100ug 100 ug
EUR 481

Casein Kinase 2 Beta (Casein Kinase 2 beta) Antibody

abx231281-100ug 100 ug
EUR 509

Recombinant Human Glutathione S-transferase kappa 1 Protein, GST, E.coli-100ug

QP6125-ec-100ug 100ug
EUR 426
The an infection induced a considerably larger arginase expression in B-1 cells, however the stimulation with EVs led to a lower on this gene expression. TLR-2 and TLR-6 had considerably larger expression in B-1 cells from mice intraperitoneally stimulated with the parasite. The TLR-9 expression was larger in animals contaminated or stimulated for 48 h with EVs. Apparently, in B-1 cells the stimulus with L. amazonensis led to a considerable improve within the expression of myeloid restricted transcription components. Thus, our research means that the parasites or EVs in another way modulated the activation and differentiation of B-1 cells.