Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation
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December 10, 2020

Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation

By Charles

Immune evasion is a vital most cancers hallmark and the understanding of its mechanisms has generated profitable therapeutic approaches. Induction of immunogenic cell demise (ICD) is anticipated to draw immune cell populations that promote innate and adaptive immune responses. Right here, we current a important advance for our adenovirus-mediated gene remedy method, the place the mixed p14ARF and human interferon-β (IFNβ) gene switch to human melanoma cells led to oncolysis, ICD and subsequent activation of immune cells. Our outcomes point out that IFNβ alone or together with p14ARF was capable of induce large cell demise within the human melanoma cell line SK-MEL-147, although caspase 3/7 activation was not important.

In situ gene remedy of s.c. SK-MEL-147 tumors in Nod-Scid mice revealed inhibition of tumor development and elevated survival in response to IFNβ alone or together with p14ARF. Emission of important markers of ICD (exposition of calreticulin, secretion of ATP and IFNβ) was stronger when cells had been handled with mixed p14ARF and IFNβ gene switch. Co-culture of beforehand transduced SK-MEL-147 cells with monocyte-derived dendritic cells (Mo-DCs) derived from wholesome donors resulted in elevated ranges of activation markers HLA-DR, CD80, and CD86.

Activated Mo-DCs had been capable of prime autologous and allogeneic T cells, leading to elevated secretion of IFNγ, TNF-α, and IL-10. Preliminary knowledge confirmed that T cells primed by Mo-DCs activated with p14ARF+IFNβ-transduced SK-MEL-147 cells had been capable of induce the lack of viability of contemporary non-transduced SK-MEL-147 cells, suggesting the induction of a particular cytotoxic inhabitants that acknowledged and killed SK-MEL-147 cells. Collectively, our outcomes point out that p14ARF and IFNβ delivered by our adenoviral system induced oncolysis in human melanoma cells accompanied by adaptive immune response activation and regulation.

 

LT-Okay63 Enhances B Cell Activation and Survival Elements in Neonatal Mice That Interprets Into Lengthy-Lived Humoral Immunity

Adjuvants improve magnitude and length of immune responses induced by vaccines. On this research we assessed in neonatal mice if and the way the adjuvant LT-Okay63 given with a pneumococcal conjugate vaccine, Pnc1-TT, may have an effect on the expression of tumor necrosis issue receptor (TNF-R) superfamily members, identified to be concerned within the initiation and upkeep of antibody responses; B cell activating issue receptor (BAFF-R) and B cell maturation antigen (BCMA) and their ligands, BAFF, and a proliferation inducing ligand (APRIL). Initially we assessed the maturation standing of various B cell populations and their expression of BAFF-R and BCMA. Neonatal mice had dramatically fewer B cells than grownup mice and the composition of various subsets throughout the B cell pool differed tremendously.
Proportionally newly fashioned B cells had been most considerable, however they’d diminished BAFF-R expression which may clarify low proportions of marginal zone and follicular B cells noticed. Restricted BCMA expression was additionally detected in neonatal pre-plasmablasts/plasmablasts. LT-Okay63 enhanced vaccine-induced BAFF-R expression in splenic marginal zone, follicular and newly fashioned B cells, resulting in elevated plasmablast/plasma cells, and their enhanced expression of BCMA in spleen and bone marrow. Moreover, the induction of BAFF and APRIL expression occurred early in neonatal mice immunized with Pnc1-TT both with or with out LT-Okay63. Nonetheless, BAFF+ and APRIL+ cells in spleens had been maintained at a better degree in mice that obtained the adjuvant. Moreover, the early improve of APRIL+ cells in bone marrow was extra profound in mice immunized with vaccine and adjuvant.
Lastly, we assessed, for the primary time in neonatal mice, accent cells of the plasma cell area of interest in bone marrow and their secretion of APRIL. We discovered that LT-Okay63 enhanced the frequency and APRIL expression of eosinophils, macrophages, and megakaryocytes, which probably contributed to plasma cell survival, regardless that APRIL+ cells confirmed a quick decline. All this was related to enhanced, sustained vaccine-specific antibody-secreting cells in bone marrow and persisting vaccine-specific serum antibodies. Our research sheds mild on the mechanisms behind the adjuvanticity of LT-Okay63 and identifies molecular pathways that must be triggered by vaccine adjuvants to induce sustained humoral immunity in formative years.
Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation

Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation

Leishmania amazonensis Promastigotes or Extracellular Vesicles Modulate B-1 Cell Activation and Differentiation

B-1 cells are thought-about an innate-like B cell inhabitants that participates in efficient innate and adaptive responses to pathogens. B-1 cells produce immunoglobulins, cytokines, chemokines, migrate to inflammatory websites, and differentiate into mononuclear phagocyte-like cells. Murine B-1 cells phagocytosed Leishmania in vitro and in vivo and take part in immunity in opposition to Leishmania. Our group confirmed that B-1 cells or their extracellular vesicles (EVs) led to a resistance to experimental an infection by L. amazonensis. Nonetheless, the B-1 cells’ responses to Leishmania or EVs remoted from parasites are nonetheless poorly characterised. Learning the activation and differentiation of B-1 cells in vivo can contribute to a greater understanding of how these cells take part in immunity to L. amazonensis.
Thus, we evaluated the expression of myeloid (M-csfr, G-csfr, Spi-1) and lymphoid (EBF, E2A, IL-7R) lineage dedication components, Toll-like receptors (TLRs), activation cell floor markers, nitric oxide (NO) and reactive oxygen species (ROS) manufacturing in murine peritoneal B-1 cells collected after 24 or 48 h post-infection with Leishmania (Leishmania) amazonensis promastigotes or EVs launched by the parasites. Our outcomes demonstrated that L. amazonensis an infection didn’t stimulate the expression of CD40, CD80, CD86, F4/80, and MHC II in B-1 cells, however a major lower within the manufacturing of NO and ROS was noticed.

Recombinant Casein Kappa (CSN3)
4-RPJ331Hu01
  • EUR 593.09
  • EUR 282.00
  • EUR 1894.08
  • EUR 711.36
  • EUR 1302.72
  • EUR 472.80
  • EUR 4555.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Casein Kappa expressed in: E.coli

Kappa casein ELISA Kit| Bovine Kappa casein ELISA Kit
EF011008 96 Tests
EUR 826.8

Casein Kappa (CSN3) Antibody
20-abx171599
  • EUR 1028.40
  • EUR 526.80
  • 1 mg
  • 200 ug

Casein Kappa (CSN3) Antibody
20-abx171600
  • EUR 1178.40
  • EUR 594.00
  • 1 mg
  • 200 ug

Human Casein Kappa (CSN3) Protein
20-abx167038
  • EUR 828.00
  • EUR 343.20
  • EUR 2548.80
  • EUR 978.00
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Cow Casein Kappa (CSN3) Protein
20-abx650845
  • EUR 944.40
  • EUR 360.00
  • EUR 2983.20
  • EUR 1128.00
  • EUR 661.20
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Casein Kappa (CSN3)ELISA Kit
201-12-2883 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Cow Casein kappa (CSN3) ELISA Kit
20-abx150084
  • EUR 9493.20
  • EUR 5058.00
  • EUR 1167.60
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Casein kappa (CSN3) ELISA Kit
20-abx150942
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Cow Casein kappa (CSN3) CLIA Kit
20-abx495684
  • EUR 11090.40
  • EUR 5904.00
  • EUR 1356.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Casein kappa (CSN3) CLIA Kit
20-abx495685
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Cow Kappa Casein (CSN3) ELISA Kit
abx555452-96tests 96 tests
EUR 1093.2

Pig Kappa Casein (CSN3) ELISA Kit
abx555666-96tests 96 tests
EUR 1093.2

Rat Kappa Casein (CSN3) ELISA Kit
abx555992-96tests 96 tests
EUR 801.6

Mouse Kappa Casein (CSN3) ELISA Kit
abx556064-96tests 96 tests
EUR 801.6

Human Kappa Casein (CSN3) ELISA Kit
abx575410-96tests 96 tests
EUR 801.6

Bovine Kappa-casein(CSN3) ELISA kit
CSB-EL006064BO-24T 1 plate of 24 wells
EUR 198
Description: Quantitativecompetitive ELISA kit for measuring Bovine Kappa-casein (CSN3) in samples from breastmilk. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Bovine Kappa-casein(CSN3) ELISA kit
1-CSB-EL006064BO
  • EUR 1080.00
  • EUR 6571.20
  • EUR 3480.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativecompetitive ELISA kit for measuring Bovine Kappa-casein(CSN3) in samples from breastmilk. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Kappa-casein(CSN3) ELISA kit
CSB-EL006064HU-24T 1 plate of 24 wells
EUR 198
Description: Quantitativecompetitive ELISA kit for measuring Human Kappa-casein (CSN3) in samples from serum, plasma, tissue homogenates, otherbiologicalfluids. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Kappa-casein(CSN3) ELISA kit
1-CSB-EL006064HU
  • EUR 964.80
  • EUR 6118.80
  • EUR 3244.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativecompetitive ELISA kit for measuring Human Kappa-casein(CSN3) in samples from serum, plasma, tissue homogenates, otherbiologicalfluids. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Bovine Casein Kappa (CSN3) ELISA Kit
DLR-CSN3-b-48T 48T
EUR 710.4
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Casein Kappa (CSN3) in samples from milk.

Bovine Casein Kappa (CSN3) ELISA Kit
DLR-CSN3-b-96T 96T
EUR 932.4
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Casein Kappa (CSN3) in samples from milk.

Human Casein Kappa (CSN3) ELISA Kit
DLR-CSN3-Hu-48T 48T
EUR 620.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Casein Kappa (CSN3) in samples from serum, plasma or other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit
DLR-CSN3-Hu-96T 96T
EUR 807.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Casein Kappa (CSN3) in samples from serum, plasma or other biological fluids.

Goat Kappa casein(CSN3) ELISA kit
E06K0085-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Kappa casein(CSN3) ELISA kit
E06K0085-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Kappa casein(CSN3) ELISA kit
E06K0085-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human CSN3/ Kappa-casein ELISA Kit
E0577Hu 1 Kit
EUR 726

Rabbit Kappa casein(CSN3) ELISA kit
E04K0085-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rabbit Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Kappa casein(CSN3) ELISA kit
E04K0085-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rabbit Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Kappa casein(CSN3) ELISA kit
E04K0085-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rabbit Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Kappa casein(CSN3) ELISA kit
E01K0085-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Kappa casein(CSN3) ELISA kit
E01K0085-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Kappa casein(CSN3) ELISA kit
E01K0085-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Bovine CSN3/ Kappa-casein ELISA Kit
E0074Bo 1 Kit
EUR 860.4

Mouse Kappa casein(CSN3) ELISA kit
E03K0085-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Kappa casein(CSN3) ELISA kit
E03K0085-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Kappa casein(CSN3) ELISA kit
E03K0085-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Mouse Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Kappa casein(CSN3) ELISA kit
E09K0085-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Monkey Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Kappa casein(CSN3) ELISA kit
E09K0085-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Monkey Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Kappa casein(CSN3) ELISA kit
E09K0085-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Monkey Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Kappa casein(CSN3) ELISA kit
E02K0085-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Kappa casein(CSN3) ELISA kit
E02K0085-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Kappa casein(CSN3) ELISA kit
E02K0085-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Kappa casein(CSN3) ELISA kit
E08K0085-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Kappa casein(CSN3) ELISA kit
E08K0085-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Kappa casein(CSN3) ELISA kit
E08K0085-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

ELISA kit for Rat Kappa-casein
EK3314 96 tests
EUR 804
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Kappa-casein in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Bovine Kappa-casein
EK3315 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Bovine Kappa-casein in samples from serum, plasma, tissue homogenates and other biological fluids.

Bovine Kappa- casein, CSN3 ELISA KIT
ELI-23848b 96 Tests
EUR 1113.6

Human Kappa- casein, CSN3 ELISA KIT
ELI-23849h 96 Tests
EUR 988.8

Porcine Kappa- casein, CSN3 ELISA KIT
ELI-23850p 96 Tests
EUR 1113.6

Rat Kappa- casein, Csn3 ELISA KIT
ELI-33106r 96 Tests
EUR 1063.2

Mouse Kappa- casein, Csn3 ELISA KIT
ELI-34476m 96 Tests
EUR 1038

Rat Csn3/ Kappa-casein ELISA Kit
E0241Ra 1 Kit
EUR 775.2

Pig Kappa casein(CSN3) ELISA kit
E07K0085-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Kappa casein(CSN3) ELISA kit
E07K0085-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Kappa casein(CSN3) ELISA kit
E07K0085-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine)
4-PAJ331Bo01
  • EUR 328.80
  • EUR 3534.00
  • EUR 868.80
  • EUR 418.80
  • EUR 271.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3)

Casein Kappa (CSN3) Polyclonal Antibody (Human)
4-PAJ331Hu01
  • EUR 296.40
  • EUR 3012.00
  • EUR 750.00
  • EUR 372.00
  • EUR 256.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3)

Cattle Casein Kappa (CSN3) ELISA Kit
SEJ331Bo-10x96wellstestplate 10x96-wells test plate
EUR 6777.36
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Casein Kappa (CSN3) in milk.

Cattle Casein Kappa (CSN3) ELISA Kit
SEJ331Bo-1x48wellstestplate 1x48-wells test plate
EUR 663.31
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Casein Kappa (CSN3) in milk.

Cattle Casein Kappa (CSN3) ELISA Kit
SEJ331Bo-1x96wellstestplate 1x96-wells test plate
EUR 896.16
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Casein Kappa (CSN3) in milk.

Cattle Casein Kappa (CSN3) ELISA Kit
SEJ331Bo-5x96wellstestplate 5x96-wells test plate
EUR 3672.72
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Casein Kappa (CSN3) in milk.

Cattle Casein Kappa (CSN3) ELISA Kit
4-SEJ331Bo
  • EUR 6837.60
  • EUR 3613.20
  • EUR 896.40
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Cattle Casein Kappa (CSN3) in samples from milk with no significant corss-reactivity with analogues from other species.

Human Casein Kappa (CSN3) ELISA Kit
SEJ331Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Casein Kappa (CSN3) in Breast milk and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit
SEJ331Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Casein Kappa (CSN3) in Breast milk and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit
SEJ331Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Casein Kappa (CSN3) in Breast milk and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit
SEJ331Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Casein Kappa (CSN3) in Breast milk and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit
4-SEJ331Hu
  • EUR 5738.40
  • EUR 3031.20
  • EUR 768.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Casein Kappa (CSN3) in samples from Breast milk and other biological fluids with no significant corss-reactivity with analogues from other species.

Bovine Casein Kappa (CSN3) ELISA Kit
RD-CSN3-b-48Tests 48 Tests
EUR 727.2

Bovine Casein Kappa (CSN3) ELISA Kit
RD-CSN3-b-96Tests 96 Tests
EUR 1012.8

Human Casein Kappa (CSN3) ELISA Kit
RD-CSN3-Hu-48Tests 48 Tests
EUR 625.2

Human Casein Kappa (CSN3) ELISA Kit
RD-CSN3-Hu-96Tests 96 Tests
EUR 867.6

Bovine Casein Kappa (CSN3) ELISA Kit
RDR-CSN3-b-48Tests 48 Tests
EUR 759.6

Bovine Casein Kappa (CSN3) ELISA Kit
RDR-CSN3-b-96Tests 96 Tests
EUR 1060.8

Human Casein Kappa (CSN3) ELISA Kit
RDR-CSN3-Hu-48Tests 48 Tests
EUR 652.8

Human Casein Kappa (CSN3) ELISA Kit
RDR-CSN3-Hu-96Tests 96 Tests
EUR 907.2

Bovine Casein Kappa ELISA Kit (CSN3)
RK00450 96 Tests
EUR 687.6

Human Casein Kappa ELISA Kit (CSN3)
RK01196 96 Tests
EUR 625.2

Human Casein Kappa(CSN3)ELISA Kit
QY-E02404 96T
EUR 433.2

Human Casein Kappa (CSN3)ELISA Kit
YLA3486HU-48T 48T
EUR 435

Human Casein Kappa (CSN3)ELISA Kit
YLA3486HU-96T 96T
EUR 562.5

Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN 50 µg
EUR 418.8

ELISA kit for Cattle CSN3 (Casein Kappa)
ELK8007 1 plate of 96 wells
EUR 631.2
Description: A sandwich ELISA kit for detection of Casein Kappa from Cattle in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Human CSN3 (Casein Kappa)
ELK4819 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Casein Kappa from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Guinea pig Kappa casein(CSN3) ELISA kit
E05K0085-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Guinea pig Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Kappa casein(CSN3) ELISA kit
E05K0085-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Guinea pig Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Kappa casein(CSN3) ELISA kit
E05K0085-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Guinea pig Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

ELISA kit for Human Kappa-casein (CSN3)
KTE62271-48T 48T
EUR 398.4
Description: Quantitative sandwich ELISA for measuring Human Kappa-casein (CSN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Kappa-casein (CSN3)
KTE62271-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Human Kappa-casein (CSN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Kappa-casein (CSN3)
KTE62271-96T 96T
EUR 646.8
Description: Quantitative sandwich ELISA for measuring Human Kappa-casein (CSN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), APC
4-PAJ331Bo01-APC
  • EUR 463.20
  • EUR 4642.80
  • EUR 1273.20
  • EUR 598.80
  • EUR 284.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with APC.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), Biotinylated
4-PAJ331Bo01-Biotin
  • EUR 410.40
  • EUR 3474.00
  • EUR 1003.20
  • EUR 508.80
  • EUR 278.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with Biotin.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), Cy3
4-PAJ331Bo01-Cy3
  • EUR 568.80
  • EUR 6140.40
  • EUR 1647.60
  • EUR 748.80
  • EUR 330.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with Cy3.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), FITC
4-PAJ331Bo01-FITC
  • EUR 394.80
  • EUR 3738.00
  • EUR 1041.60
  • EUR 502.80
  • EUR 252.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with FITC.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), HRP
4-PAJ331Bo01-HRP
  • EUR 421.20
  • EUR 4042.80
  • EUR 1123.20
  • EUR 538.80
  • EUR 266.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with HRP.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), PE
4-PAJ331Bo01-PE
  • EUR 394.80
  • EUR 3738.00
  • EUR 1041.60
  • EUR 502.80
  • EUR 252.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with PE.

Casein Kappa (CSN3) Polyclonal Antibody (Human), APC
4-PAJ331Hu01-APC
  • EUR 414.00
  • EUR 3930.00
  • EUR 1094.40
  • EUR 528.00
  • EUR 262.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with APC.

Casein Kappa (CSN3) Polyclonal Antibody (Human), Biotinylated
4-PAJ331Hu01-Biotin
  • EUR 373.20
  • EUR 2952.00
  • EUR 872.40
  • EUR 457.20
  • EUR 262.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with Biotin.

Casein Kappa (CSN3) Polyclonal Antibody (Human), Cy3
4-PAJ331Hu01-Cy3
  • EUR 502.80
  • EUR 5190.00
  • EUR 1410.00
  • EUR 654.00
  • EUR 301.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with Cy3.

Casein Kappa (CSN3) Polyclonal Antibody (Human), FITC
4-PAJ331Hu01-FITC
  • EUR 355.20
  • EUR 3168.00
  • EUR 900.00
  • EUR 446.40
  • EUR 234.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with FITC.

Casein Kappa (CSN3) Polyclonal Antibody (Human), HRP
4-PAJ331Hu01-HRP
  • EUR 379.20
  • EUR 3426.00
  • EUR 968.40
  • EUR 477.60
  • EUR 247.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with HRP.

Casein Kappa (CSN3) Polyclonal Antibody (Human), PE
4-PAJ331Hu01-PE
  • EUR 355.20
  • EUR 3168.00
  • EUR 900.00
  • EUR 446.40
  • EUR 234.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with PE.

immunoglobulin Kappa (immunoglobulin kappa) Antibody
abx234295-100ug 100 ug
EUR 610.8

Mouse Anti-Casein monoclonal antibody, clone CSD
HMABPY120D-100ug 100 ug
EUR 639.6

Recombinant Human Casein kinase I isoform epsilon Protein, GST, E.coli-100ug
QP8358-ec-100ug 100ug
EUR 489.6

Recombinant Human Casein kinase II subunit beta Protein, GST, E.coli-100ug
QP8381-ec-100ug 100ug
EUR 489.6

Recombinant Human Casein kinase I isoform epsilon Protein, His, E.coli-100ug
QP5880-ec-100ug 100ug
EUR 489.6

Recombinant Human Casein kinase I isoform epsilon Protein, His, Yeast-100ug
QP5880-ye-100ug 100ug
EUR 576

Anti-Kappa Light Chain Antibody Clone SPM508, Unconjugated-100ug
3514-MSM1X-P1 100ug
EUR 513.6

Anti-Kappa Light Chain Antibody Clone HP6053, Unconjugated-100ug
3514-MSM4-P1 100ug
EUR 513.6

Anti-Kappa Light Chain Antibody Clone KLC709, Unconjugated-100ug
3514-MSM6-P1 100ug
EUR 513.6

Anti-Kappa Light Chain Antibody Clone KLC1278, Unconjugated-100ug
3514-MSM8-P1 100ug
EUR 513.6

Csn3 ELISA Kit| Mouse Kappa-casein ELISA Kit
EF014412 96 Tests
EUR 826.8

Csn3 ELISA Kit| Rat Kappa-casein ELISA Kit
EF018435 96 Tests
EUR 826.8

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), APC-Cy7
4-PAJ331Bo01-APC-Cy7
  • EUR 783.60
  • EUR 9141.60
  • EUR 2402.40
  • EUR 1053.60
  • EUR 426.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with APC-Cy7.

Casein Kappa (CSN3) Polyclonal Antibody (Human), APC-Cy7
4-PAJ331Hu01-APC-Cy7
  • EUR 685.20
  • EUR 7716.00
  • EUR 2046.00
  • EUR 912.00
  • EUR 382.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with APC-Cy7.

anti-beta Casein
YF-PA23527 50 ul
EUR 400.8
Description: Mouse polyclonal to beta Casein

Casein Kinase 1 Delta (Casein Kinase 1 delta) Antibody
abx231279-100ug 100 ug
EUR 577.2

Casein Kinase 1 Epsilon (Casein Kinase 1 epsilon) Antibody
abx231280-100ug 100 ug
EUR 577.2
The an infection induced a considerably larger arginase expression in B-1 cells, however the stimulation with EVs led to a lower on this gene expression. TLR-2 and TLR-6 had considerably larger expression in B-1 cells from mice intraperitoneally stimulated with the parasite. The TLR-9 expression was larger in animals contaminated or stimulated for 48 h with EVs. Apparently, in B-1 cells the stimulus with L. amazonensis led to a considerable improve within the expression of myeloid restricted transcription components. Thus, our research means that the parasites or EVs in another way modulated the activation and differentiation of B-1 cells.
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