Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation
December 10, 2020

Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation

By Charles

Immune evasion is a vital most cancers hallmark and the understanding of its mechanisms has generated profitable therapeutic approaches. Induction of immunogenic cell demise (ICD) is anticipated to draw immune cell populations that promote innate and adaptive immune responses. Right here, we current a important advance for our adenovirus-mediated gene remedy method, the place the mixed p14ARF and human interferon-β (IFNβ) gene switch to human melanoma cells led to oncolysis, ICD and subsequent activation of immune cells. Our outcomes point out that IFNβ alone or together with p14ARF was capable of induce large cell demise within the human melanoma cell line SK-MEL-147, although caspase 3/7 activation was not important.

In situ gene remedy of s.c. SK-MEL-147 tumors in Nod-Scid mice revealed inhibition of tumor development and elevated survival in response to IFNβ alone or together with p14ARF. Emission of important markers of ICD (exposition of calreticulin, secretion of ATP and IFNβ) was stronger when cells had been handled with mixed p14ARF and IFNβ gene switch. Co-culture of beforehand transduced SK-MEL-147 cells with monocyte-derived dendritic cells (Mo-DCs) derived from wholesome donors resulted in elevated ranges of activation markers HLA-DR, CD80, and CD86.

Activated Mo-DCs had been capable of prime autologous and allogeneic T cells, leading to elevated secretion of IFNγ, TNF-α, and IL-10. Preliminary knowledge confirmed that T cells primed by Mo-DCs activated with p14ARF+IFNβ-transduced SK-MEL-147 cells had been capable of induce the lack of viability of contemporary non-transduced SK-MEL-147 cells, suggesting the induction of a particular cytotoxic inhabitants that acknowledged and killed SK-MEL-147 cells. Collectively, our outcomes point out that p14ARF and IFNβ delivered by our adenoviral system induced oncolysis in human melanoma cells accompanied by adaptive immune response activation and regulation.

 

LT-Okay63 Enhances B Cell Activation and Survival Elements in Neonatal Mice That Interprets Into Lengthy-Lived Humoral Immunity

Adjuvants improve magnitude and length of immune responses induced by vaccines. On this research we assessed in neonatal mice if and the way the adjuvant LT-Okay63 given with a pneumococcal conjugate vaccine, Pnc1-TT, may have an effect on the expression of tumor necrosis issue receptor (TNF-R) superfamily members, identified to be concerned within the initiation and upkeep of antibody responses; B cell activating issue receptor (BAFF-R) and B cell maturation antigen (BCMA) and their ligands, BAFF, and a proliferation inducing ligand (APRIL). Initially we assessed the maturation standing of various B cell populations and their expression of BAFF-R and BCMA. Neonatal mice had dramatically fewer B cells than grownup mice and the composition of various subsets throughout the B cell pool differed tremendously.
Proportionally newly fashioned B cells had been most considerable, however they’d diminished BAFF-R expression which may clarify low proportions of marginal zone and follicular B cells noticed. Restricted BCMA expression was additionally detected in neonatal pre-plasmablasts/plasmablasts. LT-Okay63 enhanced vaccine-induced BAFF-R expression in splenic marginal zone, follicular and newly fashioned B cells, resulting in elevated plasmablast/plasma cells, and their enhanced expression of BCMA in spleen and bone marrow. Moreover, the induction of BAFF and APRIL expression occurred early in neonatal mice immunized with Pnc1-TT both with or with out LT-Okay63. Nonetheless, BAFF+ and APRIL+ cells in spleens had been maintained at a better degree in mice that obtained the adjuvant. Moreover, the early improve of APRIL+ cells in bone marrow was extra profound in mice immunized with vaccine and adjuvant.
Lastly, we assessed, for the primary time in neonatal mice, accent cells of the plasma cell area of interest in bone marrow and their secretion of APRIL. We discovered that LT-Okay63 enhanced the frequency and APRIL expression of eosinophils, macrophages, and megakaryocytes, which probably contributed to plasma cell survival, regardless that APRIL+ cells confirmed a quick decline. All this was related to enhanced, sustained vaccine-specific antibody-secreting cells in bone marrow and persisting vaccine-specific serum antibodies. Our research sheds mild on the mechanisms behind the adjuvanticity of LT-Okay63 and identifies molecular pathways that must be triggered by vaccine adjuvants to induce sustained humoral immunity in formative years.
 Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation

Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation

Leishmania amazonensis Promastigotes or Extracellular Vesicles Modulate B-1 Cell Activation and Differentiation

B-1 cells are thought-about an innate-like B cell inhabitants that participates in efficient innate and adaptive responses to pathogens. B-1 cells produce immunoglobulins, cytokines, chemokines, migrate to inflammatory websites, and differentiate into mononuclear phagocyte-like cells. Murine B-1 cells phagocytosed Leishmania in vitro and in vivo and take part in immunity in opposition to Leishmania. Our group confirmed that B-1 cells or their extracellular vesicles (EVs) led to a resistance to experimental an infection by L. amazonensis. Nonetheless, the B-1 cells’ responses to Leishmania or EVs remoted from parasites are nonetheless poorly characterised. Learning the activation and differentiation of B-1 cells in vivo can contribute to a greater understanding of how these cells take part in immunity to L. amazonensis.
Thus, we evaluated the expression of myeloid (M-csfr, G-csfr, Spi-1) and lymphoid (EBF, E2A, IL-7R) lineage dedication components, Toll-like receptors (TLRs), activation cell floor markers, nitric oxide (NO) and reactive oxygen species (ROS) manufacturing in murine peritoneal B-1 cells collected after 24 or 48 h post-infection with Leishmania (Leishmania) amazonensis promastigotes or EVs launched by the parasites. Our outcomes demonstrated that L. amazonensis an infection didn’t stimulate the expression of CD40, CD80, CD86, F4/80, and MHC II in B-1 cells, however a major lower within the manufacturing of NO and ROS was noticed.

Casein Kappa (CSN3) Antibody

20-abx129181
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Casein Kappa (CSN3) Antibody

20-abx130922
  • EUR 577.20
  • EUR 159.60
  • EUR 1680.00
  • EUR 794.40
  • EUR 427.20
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Kappa Casein (CSN3) Antibody

abx232019-100ug 100 ug
EUR 577.2

Recombinant Casein Kappa (CSN3)

4-RPJ331Bo01
  • EUR 679.10
  • EUR 304.80
  • EUR 2216.64
  • EUR 818.88
  • EUR 1517.76
  • EUR 530.40
  • EUR 5361.60
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Bovine Casein Kappa expressed in: E.coli

Recombinant Casein Kappa (CSN3)

4-RPJ331Hu01
  • EUR 593.09
  • EUR 282.00
  • EUR 1894.08
  • EUR 711.36
  • EUR 1302.72
  • EUR 472.80
  • EUR 4555.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Casein Kappa expressed in: E.coli

Recombinant Casein Kappa (CSN3)

RPU54682-100ug 100ug
EUR 640.2

Recombinant Casein Kappa (CSN3)

RPU54682-1mg 1mg
EUR 2839.2

Recombinant Casein Kappa (CSN3)

RPU54682-50ug 50ug
EUR 514.8

Recombinant Casein Kappa (CSN3)

RPU53251-100ug 100ug
EUR 542.3

Recombinant Casein Kappa (CSN3)

RPU53251-1mg 1mg
EUR 2402.4

Recombinant Casein Kappa (CSN3)

RPU53251-50ug 50ug
EUR 435.6

Casein Kappa (CSN3) Polyclonal Antibody

CAU21739-100ul 100ul
EUR 235.2

Casein Kappa (CSN3) Polyclonal Antibody

CAU21739-200ul 200ul
EUR 294

Casein Kappa (CSN3) Polyclonal Antibody

CAU29057-100ul 100ul
EUR 279.4

Casein Kappa (CSN3) Polyclonal Antibody

CAU29057-200ul 200ul
EUR 349.4

Human Casein Kappa ELISA Kit

IHUCSN3KT each
EUR 733
Description: Human Casein Kappa ELISA Kit

Cow Casein Kappa (CSN3) Protein

20-abx650845
  • EUR 944.40
  • EUR 360.00
  • EUR 2983.20
  • EUR 1128.00
  • EUR 661.20
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Bovine Casein Kappa ELISA Kit

IBOCSN3KT each
EUR 866
Description: Bovine Casein Kappa ELISA Kit

Human Casein Kappa (CSN3) Protein

20-abx167038
  • EUR 828.00
  • EUR 343.20
  • EUR 2548.80
  • EUR 978.00
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Cow Casein kappa (CSN3) CLIA Kit

20-abx495684
  • EUR 11090.40
  • EUR 5904.00
  • EUR 1356.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Cow Casein kappa (CSN3) ELISA Kit

20-abx150084
  • EUR 9493.20
  • EUR 5058.00
  • EUR 1167.60
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Casein kappa (CSN3) CLIA Kit

20-abx495685
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Casein Kappa (CSN3) Polyclonal Antibody (Human)

4-PAJ331Hu01
  • EUR 296.40
  • EUR 3012.00
  • EUR 750.00
  • EUR 372.00
  • EUR 256.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3)

Human Casein kappa (CSN3) ELISA Kit

20-abx150942
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Casein Kappa (CSN3)ELISA Kit

201-12-2883 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

DLR-CSN3-Hu-48T 48T
EUR 620.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Casein Kappa (CSN3) in samples from serum, plasma or other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

DLR-CSN3-Hu-96T 96T
EUR 807.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Casein Kappa (CSN3) in samples from serum, plasma or other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

SEJ331Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Casein Kappa (CSN3) in Breast milk and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

SEJ331Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Casein Kappa (CSN3) in Breast milk and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

SEJ331Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Casein Kappa (CSN3) in Breast milk and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

SEJ331Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Casein Kappa (CSN3) in Breast milk and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

4-SEJ331Hu
  • EUR 5738.40
  • EUR 3031.20
  • EUR 768.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Casein Kappa (CSN3) in samples from Breast milk and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Casein Kappa ELISA Kit (CSN3)

RK01196 96 Tests
EUR 625.2

Human Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-Hu-48Tests 48 Tests
EUR 652.8

Human Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-Hu-96Tests 96 Tests
EUR 907.2

Human Casein Kappa(CSN3)ELISA Kit

QY-E02404 96T
EUR 433.2

Human Casein Kappa (CSN3) ELISA Kit

RD-CSN3-Hu-48Tests 48 Tests
EUR 625.2

Human Casein Kappa (CSN3) ELISA Kit

RD-CSN3-Hu-96Tests 96 Tests
EUR 867.6

Human Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-Hu-48T 48T
EUR 475.34
Description: serum, plasma and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-Hu-96T 96T
EUR 679.04
Description: serum, plasma and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

DL-CSN3-Hu 96T
EUR 441
Description: serum, plasma or other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

DLR-CSN3-Hu 96T
EUR 463
Description: serum, plasma or other biological fluids.

Human Casein Kappa (CSN3)ELISA Kit

YLA3486HU-48T 48T
EUR 435

Human Casein Kappa (CSN3)ELISA Kit

YLA3486HU-96T 96T
EUR 562.5

Human Casein Kappa (CSN3) ELISA Kit

RD-CSN3-Hu-48T 48T
EUR 452.7
Description: serum, plasma and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

RD-CSN3-Hu-96T 96T
EUR 646.7
Description: serum, plasma and other biological fluids.

Human Casein Kappa (CSN3) ELISA Kit

EKN44092-48T 48T
EUR 378.7

Human Casein Kappa (CSN3) ELISA Kit

EKN44092-5x96T 5x96T
EUR 2569.75

Human Casein Kappa (CSN3) ELISA Kit

EKN44092-96T 96T
EUR 541

Human Casein Kappa (CSN3) ELISA Kit

EKU02981-48T 48T
EUR 555.66

Human Casein Kappa (CSN3) ELISA Kit

EKU02981-5x96T 5x96T
EUR 3770.55

Human Casein Kappa (CSN3) ELISA Kit

EKU02981-96T 96T
EUR 793.8

Human Casein Kappa (CSN3) ELISA Kit

EKL56543-5x96T 5x96T
EUR 3793.35

Human Casein Kappa (CSN3) ELISA Kit

EKL56543-96T 96T
EUR 798.6

Human CSN3(Casein Kappa) ELISA Kit

ELK4819-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CSN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CSN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CSN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CSN3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human CSN3(Casein Kappa) ELISA Kit

ELK4819-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CSN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CSN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CSN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CSN3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine)

4-PAJ331Bo01
  • EUR 328.80
  • EUR 3534.00
  • EUR 868.80
  • EUR 418.80
  • EUR 271.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3)

Bovine Casein Kappa (CSN3) ELISA Kit

DLR-CSN3-b-48T 48T
EUR 710.4
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Casein Kappa (CSN3) in samples from milk.

Bovine Casein Kappa (CSN3) ELISA Kit

DLR-CSN3-b-96T 96T
EUR 932.4
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Casein Kappa (CSN3) in samples from milk.

Bovine Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-b-48Tests 48 Tests
EUR 759.6

Bovine Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-b-96Tests 96 Tests
EUR 1060.8

Bovine Casein Kappa ELISA Kit (CSN3)

RK00450 96 Tests
EUR 687.6

Bovine Casein Kappa (CSN3) ELISA Kit

RD-CSN3-b-48Tests 48 Tests
EUR 727.2

Bovine Casein Kappa (CSN3) ELISA Kit

RD-CSN3-b-96Tests 96 Tests
EUR 1012.8

Bovine Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-b-48T 48T
EUR 561.02
Description: milk.

Bovine Casein Kappa (CSN3) ELISA Kit

RDR-CSN3-b-96T 96T
EUR 801.47
Description: milk.

Bovine Casein Kappa (CSN3) ELISA Kit

DL-CSN3-b 96T
EUR 521
Description: milk.

Bovine Casein Kappa (CSN3) ELISA Kit

DLR-CSN3-b 96T
EUR 547
Description: milk.

Bovine Casein Kappa (CSN3) ELISA Kit

RD-CSN3-b-48T 48T
EUR 534.3
Description: milk.

Bovine Casein Kappa (CSN3) ELISA Kit

RD-CSN3-b-96T 96T
EUR 763.3
Description: milk.

Bovine Casein Kappa (CSN3) ELISA Kit

EKN44091-48T 48T
EUR 454.72

Bovine Casein Kappa (CSN3) ELISA Kit

EKN44091-5x96T 5x96T
EUR 3085.6

Bovine Casein Kappa (CSN3) ELISA Kit

EKN44091-96T 96T
EUR 649.6

Cattle Casein Kappa (CSN3) ELISA Kit

SEJ331Bo-10x96wellstestplate 10x96-wells test plate
EUR 6777.36
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Casein Kappa (CSN3) in milk.

Cattle Casein Kappa (CSN3) ELISA Kit

SEJ331Bo-1x48wellstestplate 1x48-wells test plate
EUR 663.31
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Casein Kappa (CSN3) in milk.

Cattle Casein Kappa (CSN3) ELISA Kit

SEJ331Bo-1x96wellstestplate 1x96-wells test plate
EUR 896.16
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Casein Kappa (CSN3) in milk.

Cattle Casein Kappa (CSN3) ELISA Kit

SEJ331Bo-5x96wellstestplate 5x96-wells test plate
EUR 3672.72
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Casein Kappa (CSN3) in milk.

Cattle Casein Kappa (CSN3) ELISA Kit

4-SEJ331Bo
  • EUR 6837.60
  • EUR 3613.20
  • EUR 896.40
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Cattle Casein Kappa (CSN3) in samples from milk with no significant corss-reactivity with analogues from other species.

Bovine Casein Kappa (CSN3) ELISA Kit

SL0215Bo - Ask for price

Bovine Casein Kappa (CSN3) ELISA Kit

EKU02980-48T 48T
EUR 666.82

Bovine Casein Kappa (CSN3) ELISA Kit

EKU02980-5x96T 5x96T
EUR 4524.85

Bovine Casein Kappa (CSN3) ELISA Kit

EKU02980-96T 96T
EUR 952.6

Cattle CSN3(Casein Kappa) ELISA Kit

ELK8007-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle CSN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle CSN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle CSN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle CSN3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Cattle CSN3(Casein Kappa) ELISA Kit

ELK8007-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle CSN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle CSN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle CSN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle CSN3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Casein Kappa (CSN3) Polyclonal Antibody (Human), PE

4-PAJ331Hu01-PE
  • EUR 355.20
  • EUR 3168.00
  • EUR 900.00
  • EUR 446.40
  • EUR 234.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with PE.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), PE

4-PAJ331Bo01-PE
  • EUR 394.80
  • EUR 3738.00
  • EUR 1041.60
  • EUR 502.80
  • EUR 252.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with PE.

Casein Kappa (CSN3) Polyclonal Antibody (Human), APC

4-PAJ331Hu01-APC
  • EUR 414.00
  • EUR 3930.00
  • EUR 1094.40
  • EUR 528.00
  • EUR 262.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with APC.

Casein Kappa (CSN3) Polyclonal Antibody (Human), Cy3

4-PAJ331Hu01-Cy3
  • EUR 502.80
  • EUR 5190.00
  • EUR 1410.00
  • EUR 654.00
  • EUR 301.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with Cy3.

Casein Kappa (CSN3) Polyclonal Antibody (Human), HRP

4-PAJ331Hu01-HRP
  • EUR 379.20
  • EUR 3426.00
  • EUR 968.40
  • EUR 477.60
  • EUR 247.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with HRP.

ELISA kit for Human CSN3 (Casein Kappa)

ELK4819 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Casein Kappa from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Cow Kappa Casein (CSN3) ELISA Kit

abx555452-96tests 96 tests
EUR 1093.2

Pig Kappa Casein (CSN3) ELISA Kit

abx555666-96tests 96 tests
EUR 1093.2

Rat Kappa Casein (CSN3) ELISA Kit

abx555992-96tests 96 tests
EUR 801.6

Dog Kappa casein(CSN3) ELISA kit

E08K0085-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Kappa casein(CSN3) ELISA kit

E08K0085-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Kappa casein(CSN3) ELISA kit

E08K0085-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Kappa casein(CSN3) ELISA kit

E02K0085-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Kappa casein(CSN3) ELISA kit

E02K0085-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Kappa casein(CSN3) ELISA kit

E02K0085-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Kappa casein(CSN3) ELISA kit

E07K0085-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Kappa casein(CSN3) ELISA kit

E07K0085-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Kappa casein(CSN3) ELISA kit

E07K0085-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Kappa- casein, Csn3 ELISA KIT

ELI-33106r 96 Tests
EUR 1063.2

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), APC

4-PAJ331Bo01-APC
  • EUR 463.20
  • EUR 4642.80
  • EUR 1273.20
  • EUR 598.80
  • EUR 284.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with APC.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), Cy3

4-PAJ331Bo01-Cy3
  • EUR 568.80
  • EUR 6140.40
  • EUR 1647.60
  • EUR 748.80
  • EUR 330.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with Cy3.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), HRP

4-PAJ331Bo01-HRP
  • EUR 421.20
  • EUR 4042.80
  • EUR 1123.20
  • EUR 538.80
  • EUR 266.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with HRP.

Casein Kappa (CSN3) Polyclonal Antibody (Human), FITC

4-PAJ331Hu01-FITC
  • EUR 355.20
  • EUR 3168.00
  • EUR 900.00
  • EUR 446.40
  • EUR 234.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Casein Kappa (CSN3). This antibody is labeled with FITC.

ELISA kit for Cattle CSN3 (Casein Kappa)

ELK8007 1 plate of 96 wells
EUR 631.2
Description: A sandwich ELISA kit for detection of Casein Kappa from Cattle in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Casein Kappa (CSN3) Polyclonal Antibody (Bovine), FITC

4-PAJ331Bo01-FITC
  • EUR 394.80
  • EUR 3738.00
  • EUR 1041.60
  • EUR 502.80
  • EUR 252.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Casein Kappa (CSN3). This antibody is labeled with FITC.

Goat Kappa casein(CSN3) ELISA kit

E06K0085-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Kappa casein(CSN3) ELISA kit

E06K0085-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Kappa casein(CSN3) ELISA kit

E06K0085-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Kappa Casein (CSN3) ELISA Kit

abx575410-96tests 96 tests
EUR 801.6

Mouse Kappa Casein (CSN3) ELISA Kit

abx556064-96tests 96 tests
EUR 801.6

Mouse Kappa casein(CSN3) ELISA kit

E03K0085-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Kappa casein(CSN3) ELISA kit

E03K0085-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Kappa casein(CSN3) ELISA kit

E03K0085-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Mouse Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Kappa casein(CSN3) ELISA kit

E01K0085-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Kappa casein(CSN3) ELISA kit

E01K0085-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Kappa casein(CSN3) ELISA kit

E01K0085-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human Kappa casein(CSN3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Kappa- casein, Csn3 ELISA KIT

ELI-34476m 96 Tests
EUR 1038
The an infection induced a considerably larger arginase expression in B-1 cells, however the stimulation with EVs led to a lower on this gene expression. TLR-2 and TLR-6 had considerably larger expression in B-1 cells from mice intraperitoneally stimulated with the parasite. The TLR-9 expression was larger in animals contaminated or stimulated for 48 h with EVs. Apparently, in B-1 cells the stimulus with L. amazonensis led to a considerable improve within the expression of myeloid restricted transcription components. Thus, our research means that the parasites or EVs in another way modulated the activation and differentiation of B-1 cells.